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The Chromatogram Series: Dextran

29 August 2013 No Comment

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This is a screenshot of the OmniSEC profile of dextran. We offer a set of broad Dextran standards for light scattering and triple detection. The run conditions are detailed in the table below.

Run Buffer PBS
Flow Rate [mL/min] 0.700
Column Set 2 x A6000M
Column Temp (°C) 35.0
Injection volume μL 100.0
Concentration [mg/mL] 2.97
Mw Weight Average [Da] 73,123
Sample dn/dc [mL/g] 0.137
Sample IV [dL/g] 0.236
Chromatogram of Dextran: Refractive Index (RI), Viscosity, Right Angle Light Scattering (RALS) signals versus elution volume.

Chromatogram of Dextran: Refractive Index (RI), Viscosity, Right Angle Light Scattering (RALS) signals versus elution volume.

Dextran is a typical polysaccharide and chromatographs very well. This dextran has a broad polydispersity index PDI = Mw/Mn value of 1.38.  It is typically used as a system suitability sample for aqueous polymer applications, and often also a good choice for system calibration.

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If you have any questions, please email me at ulf.nobbmann@malvern.com. Thanks!