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Duncan Griffiths

Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Tech Talk »

[23 Nov 2017 | ]

Results of a multi-round interlaboratory comparison (ILC) of NanoSight instruments were recently published1, setting a benchmark for repeatability and reproducibility for the Nanoparticle Tracking Analysis (NTA) technique. The limits of measurement were shown to extend beyond the 30 nm –600 nm range (with all NanoSight models), with percentage coefficients of variation (% CV), calculated as sub 5% for monodisperse samples. The process further highlighted how much variability or error might be introduced if inappropriate instrument settings or analytical procedures are used.
As a relatively new technique, there remained questions about the …

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Meet the Experts, Nanoparticle Tracking Analysis, Nanosight, Particle concentration »

[24 Aug 2017 | ]

I recently presented a webinar titled “Basic Principles of Nanoparticle Tracking Analysis: Size, Concentration and Speciation with Single-Particle Resolution”. We got such a good turnout that we were not able to address the numerous questions asked during the event.
Thank you for the participation!

Since there was such strong interest, we have provided the following list of questions and answers to benefit all who are interested.
Q: In DLS also we can get lognormal number distribution. How is the NTA number distribution different?
NTA yields a number weighted distribution because it is a particle …

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Customer experience, Nanoparticle Tracking Analysis, Nanosight, Particle concentration »

[17 Aug 2017 | ]

Being involved with the NanoSight products gives us visibility over a wide range of research areas that have a direct benefit to science and humanity. It is quite exciting to feel we are contributing to advancing science in these fields.
I was prompted to do a quick review of recent publications to see where NanoSight systems are being used after reading a recent article by Lara Aqrawi on characterizing extracellular vesicles in tears and saliva as a non-invasive diagnostic method for primary Sjögren’s syndrome (pSS) patients. Nanoparticle tracking analysis was conducted …

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Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle size, Protein Aggregation, Tech Talk »

[26 May 2016 | ]

We recently published our application note, “Adeno-Associated Virus titer and aggregation characterization“, which discusses a new NanoSight method that allows the visualization and therefore analysis of viruses smaller than what was previously possible to measure.  This represents an exciting extension of the Nanoparticle Tracking Analysis (NTA) technique in response to market demand for tools for better characterization of small virus types.
 
Adeno-Associated Virus (AAV) is commonly used in the gene therapy field as a delivery vector. Tools to characterize both the concentration and purity or aggregation of the samples are limited because the primary virus size is …

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