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Label-free analysis

Studying molecules in their native state.

Differential Scanning Calorimetry, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Protein higher order structure »

[1 Mar 2017 | ]

The NEW MicroCal PEAQ-DSC – the gold standard for thermal stability determination
Differential scanning calorimetry (DSC) is an established biophysical tool for protein stability characterization during drug development. DSC data are used to advance the most stable and ‘developable’ proteins into the pipeline, and optimize the process and formulation conditions which will maintain protein stability during manufacturing and storage. DSC data are also important in higher order structure (HOS) protein characterization, lot-to-lot comparability, and characterization of biosimilar drugs compared to innovators.
Our previous technology, the MicroCal VP-Capillary DSC Automated system, is a …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Events, Meet the Experts, MicroCal, Microcalorimetry, Protein higher order structure »

[2 Feb 2017 | ]

We are proud to announce Malvern Instruments’s Gold Sponsorship of Global Engage’s Biosimilars and Biologics Congress 2017.  This two-day event will be taking place on Monday 6th and Tuesday 7th March 2017, at the Leonardo Royal Hotel Berlin Alexanderplatz.
This meeting will be attended by industry, regulatory and academic experts from all areas of protein, antibody and biosimilar research and will comprise three topic streams: Innovations in Antibody-based Therapeutics; Expression, Purification and Characterization (with a particular focus on product stability and half-life); Biosimilars.
Natalia Markova, Principal Scientist, Malvern MicroCal, will be discussing …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Events, Meet the Experts, MicroCal, Microcalorimetry, Protein higher order structure »

[31 Jan 2017 | ]

DSC for the regulated environment
 

 
Advancing biotherapeutic molecules along the drug development pipeline is a complex, time-consuming and expensive process. Good stability is key to a candidate’s progression, and must be assessed and reviewed throughout development – from candidate selection, through formulation development, all the way to batch-to-batch comparability or biosimilarity assessment….and beyond! Objective, reliable stability data is needed for confident decision-making about which drug products and processes to move through development into commercialization.
Differential Scanning Calorimetry (DSC) is a biophysical tool for protein stability characterization. The MicroCal VP-Capillary DSC system, launched in 2002, …

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Binding affinity and Kd, Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Protein higher order structure »

[23 Aug 2016 | ]

Malvern Instruments is sponsoring two “MicroCal User Days” in October:

Tuesday October 18 2016: West Coast MicroCal User Day in San Diego, CA
This complimentary 1-day event will include presentations by scientists discussing how ITC and/or DSC are used as part of their research.

Invited speakers:
Bill Callahan III, Ph.D., Senior Scientist, Amgen Thousand Oaks, Characterizing Amorphous Suspensions: Potential Formulation Option for Monoclonal Antibodies
Matthew McGann, Product Manager, Innovative Products, Malvern Instruments, Assessing Bioformulation Stability – From prediction to characterizing particle content

Thursday October 27 2016: New England MicroCal User Day in Cambridge, MA
Come hear from …

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Differential Scanning Calorimetry, Electrophoretic light scattering, Isothermal Titration Calorimetry, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Molecular structure, Protein Aggregation, Raman spectroscopy, Zetasizer »

[12 May 2016 | ]

I had the pleasure of participating in the 5th International Symposium on Higher Order Structure of Protein Therapeutics, also known as HOS2016, sponsored by CASSS. The meeting was at the Renaissance Hotel in Long Beach, California April 11-13, 2016. I was joined by my Malvern colleagues Rajib Ahmed and David Sanborn.
The HOS Symposium is a platform for open discussion of development and optimization of analytical techniques to study HOS of proteins and biopharmaceutical products (including monoclonal antibodies and antibody-drug conjugates), biocomparability, and biosimilars.
Protein higher order structure (HOS) includes the secondary, …

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Label-free analysis, Malvern Events, Molecular weight, Spraytec »

[21 Apr 2016 | ]

“I’m looking to study the aggregation kinetics of my nanoparticles using the Zetasizer Nano ZS.
Is there a way to make time-resolved size measurements using this instrument?”
The Zetasizer has become a fairly standard sizing tool for nanoparticles, as the size and the size distribution of particles and molecules in solution can be assessed within minutes using Dynamic Light Scattering (DLS). If samples are not stable, results will not be consistent or repeatable (which would be flagged by the trend analysis within the Expert Advice tab); however, DLS can then be used to provide insight into …

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Isothermal Titration Calorimetry, Label-free analysis, Meet the Experts, MicroCal »

[18 Feb 2016 | ]

Isothermal Titration Calorimetry: An essential tool for binding affinity measurements in life sciences research
What is Isothermal Titration Calorimetry?

Isothermal titration calorimetry (ITC) is established in biophysical characterization circles as the “gold standard” for measuring binding affinity interactions involving proteins, lipids, nucleic acids and other biopolymers.
Since there are so many ways to measure binding affinity, why has Isothermal Titration Calorimetry (ITC ) become the gold standard?
ITC (Isothermal Titration Calorimetry) is a direct, label-free binding assay that measures the heat change due to a binding interaction. Every binding event and chemical process gives off heat (exothermic) …

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Isothermal Titration Calorimetry, Label-free analysis, MicroCal, Microcalorimetry, Tech Talk »

[23 Apr 2015 | ]

Don’t throw away your “bad” N-value ITC data
The N-value commonly referred as the stoichiometry is often used as a qualifier for a “good” or “bad” titration. The reasoning goes like this – “if my N-value is 20% or more different from the expected stoichiometry (often 1) then my titration results are invalid”. In reality this is not always the case. It is important to understand that the ITC N–value is not the same thing as the stoichiometry (binding ratio). N-value does in fact equal the stoichiometry if the concentrations we …

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Isothermal Titration Calorimetry, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Tech Talk »

[19 Mar 2015 | ]

We were very excited to launch PEAQ-ITC – our brand new range of Isothermal Titration Calorimetry (ITC) instruments – a couple of weeks ago with a live demonstration.  If you missed the online event, please click here to play it back.
During the Q&A session at the event, we were thrilled to receive so many questions about the new instrument.  We covered some of the questions on the day but not all of them, so this blog post addresses everything to make sure you get all the information you need. If you have a question we haven’t covered in …

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Isothermal Titration Calorimetry, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Tech Talk »

[26 Feb 2015 | ]

1977 was a memorable year for many reasons – the first Apple II computers went on sale, the first MRI Scanner was tested, and for many the stand out event was the release of the first Star Wars movie.  What you may not know is that this was also the year that MicroCal was founded by Dr. John F. Brandts.  38 years on, MicroCal has joined the Malvern family and is on the verge of launching a new range, PEAQ-ITC.  But more on that later….
Now John was an enterprising man, …

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