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Microcalorimetry

Characterizing the interactions and stability of biomolecules.

Microcalorimetry is used to study reactions involving biomolecules, including interactions between molecules and conformational changes such as protein folding. Applications range from confirming intended binding targets in small molecule drug discovery to the development of stable biotherapeutics.

Binding affinity and Kd, Isothermal Titration Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[12 Oct 2017 | ]

Isothermal Titration Calorimetry
Molecular recognition is key in all biological processes and cellular responses. It is critical to characterize the structural and thermodynamic forces behind the recognition and specificity of macromolecular interactions in order to understand the molecular events in a cell. Several biophysical techniques (including fluorescence, circular dichroism, Fourier transform infrared spectroscopy, mass spectroscopy, surface plasmon resonance, and microscale thermophoresis) are used to characterize non-covalent molecular interactions.
Isothermal titration calorimetry (ITC) has become a popular and versatile bioanalytical method for the direct measurement of binding affinity between interacting molecules.  ITC instruments were …

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Archimedes, Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Image Analysis, Label-free analysis, MicroCal, Microcalorimetry, Morphologi G3-ID, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Rheology, Size Exclusion Chromatography, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Viscotek, Zetasizer »

[21 Sep 2017 | ]

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was a range of scientists and researchers from all over the world, with one common subject in mind: Protein Stability.

One of the highlights of this conference was the pre-conference workshop. This was a free workshop for all attendees of the conference, which was sponsored by Malvern Panalytical. The workshop included a range …

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Differential Scanning Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[4 Jul 2017 | ]

Protein Antigens and DSC

This blog highlights two recent articles that focus on the use of a MicroCal Differential Scanning Calorimetry (DSC) system.
Differential Scanning Calorimetry – A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
I.B. Duruwoju, K.M. Bhandal, J. Hu, B. Carpick, and M. Kirkitadze
Journal of Visualized Experiments (JoVE), Issue 121, e55262, doi:10.3791/55262 (2017)
The authors (from Sanofi Pasteur in Toronto, Canada), provide a detailed protocol to perform Differential Scanning Calorimetry experiments on an automated DSC, such as a MicroCal VP-Capillary DSC system from Malvern Instruments.  This piece is a JoVE …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, MicroCal, Microcalorimetry, Molecular weight, Tech Talk »

[23 May 2017 | ]

A professional and personal view
If your newsfeed from scientific and mainstream media sources is anything like mine, you have seen numerous articles and blogs about the ‘reproducibility crisis’ in the life sciences.
Well-established cornerstones of scientific inquiry and research are the publication of findings in peer-reviewed journals, along with the opportunity for others to reproduce and verify published data.  This advancement and consolidation of knowledge leads to further understanding on how biological systems work, and provides the basis for the discovery and development of new drugs which benefit all of us.
However, there …

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Archimedes, Chemical identification, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry, Molecular size, Molecular structure, Molecular weight, Morphologi G3-ID, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Protein Mobility, Viscosizer TD, Zeta potential, Zetasizer »

[16 May 2017 | ]

With Pre-Conference Workshop on Biophysical Characterization and Particle Analysis
 

Date: July 17th – 20th.
We at Malvern know that protein stability is important in numerous disciplines, ranging from basic and medical biochemistry to pharmaceutical sciences. However, it is rare that researchers from all the relevant areas can join to discuss the critical issues in the field. The 2017 Colorado Protein Stability Conference provides a unique forum for this exchange of information. The topics to be covered include protein folding, dynamics and thermodynamic stability; amyloidosis and protein aggregation; and stability, formulation, analysis and processing …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[11 May 2017 | ]

In September 2016 Malvern Instruments co-organized a European MicroCal meeting with Institut Pasteur and ARBRE European molecular biophysics network. The meeting was very successful and sparked animated discussions during scientific presentations, panel and poster sessions and interactive workshops.  A key focus was on best practice for experimental design and data analysis to ensure high-quality data and informed decisions that help drive scientific projects forward.
In two days, together with more than 120 participants from 19 countries, we covered a wide range of topics, from interaction and kinetic analysis with ITC to …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry »

[1 Mar 2017 | ]

The NEW MicroCal PEAQ-DSC – the gold standard for thermal stability determination
Differential scanning calorimetry (DSC) is an established biophysical tool for protein stability characterization during drug development. DSC data are used to advance the most stable and ‘developable’ proteins into the pipeline, and optimize the process and formulation conditions which will maintain protein stability during manufacturing and storage. DSC data are also important in higher order structure (HOS) protein characterization, lot-to-lot comparability, and characterization of biosimilar drugs compared to innovators.
Our previous technology, the MicroCal VP-Capillary DSC Automated system, is a …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry »

[2 Feb 2017 | ]

We are proud to announce Malvern Instruments’s Gold Sponsorship of Global Engage’s Biosimilars and Biologics Congress 2017.  This two-day event will be taking place on Monday 6th and Tuesday 7th March 2017, at the Leonardo Royal Hotel Berlin Alexanderplatz.
This meeting will be attended by industry, regulatory and academic experts from all areas of protein, antibody and biosimilar research and will comprise three topic streams: Innovations in Antibody-based Therapeutics; Expression, Purification and Characterization (with a particular focus on product stability and half-life); Biosimilars.
Natalia Markova, Principal Scientist, Malvern MicroCal, will be discussing …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry »

[31 Jan 2017 | ]

DSC for the regulated environment
 

 
Advancing biotherapeutic molecules along the drug development pipeline is a complex, time-consuming and expensive process. Good stability is key to a candidate’s progression, and must be assessed and reviewed throughout development – from candidate selection, through formulation development, all the way to batch-to-batch comparability or biosimilarity assessment….and beyond! Objective, reliable stability data is needed for confident decision-making about which drug products and processes to move through development into commercialization.
Differential Scanning Calorimetry (DSC) is a biophysical tool for protein stability characterization. The MicroCal VP-Capillary DSC system, launched in 2002, …

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Isothermal Titration Calorimetry, MicroCal, Microcalorimetry »

[13 Dec 2016 | ]

Ah, the holidays are here! This is the time of year when we enjoy special ‘once a year’ holiday meals and treats with family and friends. There are the succulent cuts of meat, marbled with fat…..perhaps you have a holiday goose or duck. There are the vegetables and pastas coated with butter, cheese, or cream sauces. There are the rich, buttery desserts. Let’s not forget the candy, chips, eggnog, and almost every delicious, decadent item we eat or drink. We all have our favorite holiday dishes, which are likely high …

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Binding affinity and Kd, Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry »

[23 Aug 2016 | ]

Malvern Instruments is sponsoring two “MicroCal User Days” in October:

Tuesday October 18 2016: West Coast MicroCal User Day in San Diego, CA
This complimentary 1-day event will include presentations by scientists discussing how ITC and/or DSC are used as part of their research.

Invited speakers:
Bill Callahan III, Ph.D., Senior Scientist, Amgen Thousand Oaks, Characterizing Amorphous Suspensions: Potential Formulation Option for Monoclonal Antibodies
Matthew McGann, Product Manager, Innovative Products, Malvern Instruments, Assessing Bioformulation Stability – From prediction to characterizing particle content

Thursday October 27 2016: New England MicroCal User Day in Cambridge, MA
Come hear from …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry »

[29 Jul 2016 | ]

In keeping with our long tradition of working hand-in-hand with our customers, Malvern MicroCal is arranging the second of our annual European meetings to bring together current calorimetry users with all those interested in ITC and DSC technologies and applications – this will take place on September 26th-27th in Paris.
This year we are pleased to be co-organizing the meeting with Institut Pasteur (who will be hosting the meeting) and ARBRE European molecular biophysics network.
 
The agenda of the meeting encompasses a wide range of calorimetry application areas, from interaction and kinetic analysis …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Panalytical Events, MicroCal, Microcalorimetry »

[31 May 2016 | ]

Microcalorimetry is hot in Brazil!
In February 2016, I participated in microcalorimetry workshops in Rio de Janeiro and Sao Carlos, Brazil. There is a long-standing and active group of calorimetry users in Brazil, and I had the opportunity to give introductory isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) presentations at each workshop. Faculty members and students presented their research projects including ITC and/or DSC results.
 
 
These workshops were coordinated by my Malvern Brazil colleague, Henrique Kajiyama. During our Brazilian tour, Henrique and I had the opportunity to speak to attendees …

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Differential Scanning Calorimetry, Electrophoretic light scattering, Isothermal Titration Calorimetry, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry, Molecular structure, Protein Aggregation, Raman spectroscopy, Zetasizer »

[12 May 2016 | ]

I had the pleasure of participating in the 5th International Symposium on Higher Order Structure of Protein Therapeutics, also known as HOS2016, sponsored by CASSS. The meeting was at the Renaissance Hotel in Long Beach, California April 11-13, 2016. I was joined by my Malvern colleagues Rajib Ahmed and David Sanborn.
The HOS Symposium is a platform for open discussion of development and optimization of analytical techniques to study HOS of proteins and biopharmaceutical products (including monoclonal antibodies and antibody-drug conjugates), biocomparability, and biosimilars.
Protein higher order structure (HOS) includes the secondary, …

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MicroCal, Microcalorimetry »

[17 Dec 2015 | ]

 
Improve data quality and reduce downtime by taking care of the small things!  It is easily done, with a few simple steps on your part. These short videos below demonstrate step by step instructions on how to maintain your instrument and keep it in tip top shape!
All the videos in this maintenance series were developed for the user as part of a good preventive maintenance regime.
 

 
MicroCal ITC200

Installing a titration syringe
Cleaning the titration syringe
Replacing the pipette
Replacing the plunger tip

MicroCal Auto-ITC200

Installing a titration syringe
Cleaning the titration syringe
Replacing the pipette
Replacing the plunger tip

MicroCal VP-Capillary …

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Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry »

[15 Dec 2015 | ]

It was great to see so many customers in Munich for the European MicroCal User Meeting. People traveled from all over the continent for what turned out to be a very informative and informal meeting.

Top (from left to right): Prof. Gerhard Klebe (University of Marburg), Dr. Ansgar Wegener (Merck), Lukas Milles M.Sc. (LMU)
Bottom (from left to right): Dr. Eric Ennifar (University of Strasbourg), Prof. Sandro Keller (University of Kaiserslautern), Dr Barbara Zambelli (University of Bologna)
We had talks on a wide range of applications from groups studying biological pathways through to …

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Differential Scanning Calorimetry, MicroCal, Microcalorimetry »

[17 Nov 2015 | ]

We can extract multiple thermodynamic parameters from DSC data, such as ΔH, ΔHvH, ΔCp, and ΔG … but the most widely used parameter is Tm. Incidentally this is also the easiest and most accurate value to obtain – it is simply the temperature at the peak maximum.
DSC protein stability
How does it relate to protein stability?
There is more than one definition of “protein stability”. Most commonly, for industrially important proteins, the term refers to their functional (or operational) stability at physiological temperature; i.e. how long they can perform their function at 37°C? …

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Differential Scanning Calorimetry, Français, Isothermal Titration Calorimetry, MicroCal, Microcalorimetry »

[2 Jun 2015 | ]

Nous organisons des journées de microcalorimétrie très bientôt près de chez vous.
La participation à ces événements est gratuite sur inscription seulement.
Inscrivez-vous dès aujourd’hui dans les villes suivantes:

Jeudi 18 juin à Strasbourg
IMBC
Présentations principales:
– “La microcalorimétrie comme outil de caractérisation pour la biologie structurale”
Eric Ennifar de l’IBMC
– “Analyse des données d’ITC pour étude cinétique”
Philippe Dumas de l’IBMC
Je m’inscris
 

Vendredi 19 juin à Grenoble
CERMAV
Présentations principales:
– “Interactions multivalentes par ITC”
Anne Imberty du CERMAV de Grenoble
– “Caractérisation de la voie de signalisation de l’acide abscissique”
Florine Dupeux de l’Institut de Biologie Structurale à Grenoble (IBS)
Je m’inscris
 

Mercredi 24 juin à Marseille
Faculté de Pharmacie – Aix …

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Isothermal Titration Calorimetry, Label-free analysis, MicroCal, Microcalorimetry, Tech Talk »

[23 Apr 2015 | ]

Don’t throw away your “bad” N-value ITC data
The N-value commonly referred as the stoichiometry is often used as a qualifier for a “good” or “bad” titration. The reasoning goes like this – “if my N-value is 20% or more different from the expected stoichiometry (often 1) then my titration results are invalid”. In reality this is not always the case. It is important to understand that the ITC N–value is not the same thing as the stoichiometry (binding ratio). N-value does in fact equal the stoichiometry if the concentrations we …

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Differential Scanning Calorimetry, MicroCal, Microcalorimetry, Tech Talk »

[2 Apr 2015 | ]

During the development of proteins for use as biotherapeutics, the primary structure (amino acid sequence) is important in defining protein activity. It is also crucial to characterize the protein’s higher-order structure (HOS) to understand the protein’s stability, folding, structure, and functional activity.  This information is used to make decisions which drugs to move forward in development, and also as quality control and biocomparability studies.
A recently published book, Biophysical Characterization of Proteins in Developing Biopharmaceuticals (Houde and Berkowitz, authors, Elsevier, 2014), aims to help scientists successfully implement biophysical methodologies to characterize …

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