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Particle concentration

Simultaneous measurement of particle concentration and size.

Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Tech Talk »

[23 Nov 2017 | ]

Results of a multi-round interlaboratory comparison (ILC) of NanoSight instruments were recently published1, setting a benchmark for repeatability and reproducibility for the Nanoparticle Tracking Analysis (NTA) technique. The limits of measurement were shown to extend beyond the 30 nm –600 nm range (with all NanoSight models), with percentage coefficients of variation (% CV), calculated as sub 5% for monodisperse samples. The process further highlighted how much variability or error might be introduced if inappropriate instrument settings or analytical procedures are used.
As a relatively new technique, there remained questions about the …

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Archimedes, Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Image Analysis, Label-free analysis, MicroCal, Microcalorimetry, Morphologi G3-ID, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Rheology, Size Exclusion Chromatography, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Viscotek, Zetasizer »

[21 Sep 2017 | ]

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was a range of scientists and researchers from all over the world, with one common subject in mind: Protein Stability.

One of the highlights of this conference was the pre-conference workshop. This was a free workshop for all attendees of the conference, which was sponsored by Malvern Panalytical. The workshop included a range …

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Meet the Experts, Nanoparticle Tracking Analysis, Nanosight, Particle concentration »

[24 Aug 2017 | ]

I recently presented a webinar titled “Basic Principles of Nanoparticle Tracking Analysis: Size, Concentration and Speciation with Single-Particle Resolution”. We got such a good turnout that we were not able to address the numerous questions asked during the event.
Thank you for the participation!

Since there was such strong interest, we have provided the following list of questions and answers to benefit all who are interested.
Q: In DLS also we can get lognormal number distribution. How is the NTA number distribution different?
NTA yields a number weighted distribution because it is a particle …

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Customer experience, Nanoparticle Tracking Analysis, Nanosight, Particle concentration »

[17 Aug 2017 | ]

Being involved with the NanoSight products gives us visibility over a wide range of research areas that have a direct benefit to science and humanity. It is quite exciting to feel we are contributing to advancing science in these fields.
I was prompted to do a quick review of recent publications to see where NanoSight systems are being used after reading a recent article by Lara Aqrawi on characterizing extracellular vesicles in tears and saliva as a non-invasive diagnostic method for primary Sjögren’s syndrome (pSS) patients. Nanoparticle tracking analysis was conducted …

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Differential Scanning Calorimetry, Dynamic Light Scattering, Meet the Experts, MicroCal, Molecular size, Molecular weight, OMNISEC, Particle concentration, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Zetasizer »

[18 Jul 2017 | ]

Monoclonal Antibodies – or mAbs for short

Since many of these mAbs require refrigeration we had used dynamic light scattering (with a Zetasizer) to show the formation of aggregates after freeze-thaw cycles (for details see application note “Effect of storage conditions on Immunoglobulin“). These could be seen both in the increased polydispersity index (PDI) and the appearance of a large-size aggregate peak in the intensity size distribution as shown to the right. The area under the peak gives an indication of the presence of the large aggregates but is not suited for …

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Archimedes, Chemical identification, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry, Molecular size, Molecular structure, Molecular weight, Morphologi G3-ID, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Protein Mobility, Viscosizer TD, Zeta potential, Zetasizer »

[16 May 2017 | ]

With Pre-Conference Workshop on Biophysical Characterization and Particle Analysis
 

Date: July 17th – 20th.
We at Malvern know that protein stability is important in numerous disciplines, ranging from basic and medical biochemistry to pharmaceutical sciences. However, it is rare that researchers from all the relevant areas can join to discuss the critical issues in the field. The 2017 Colorado Protein Stability Conference provides a unique forum for this exchange of information. The topics to be covered include protein folding, dynamics and thermodynamic stability; amyloidosis and protein aggregation; and stability, formulation, analysis and processing …

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Dynamic Light Scattering, Molecular size, Particle concentration, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[7 Mar 2017 | ]

How can I use DLS for sample xyz?
What sample concentration / refractive index is needed for reliable DLS size measurements?

Has it been done before?
In Dynamic Light Scattering (DLS) intensity fluctuations in the sample are analyzed to obtain size information on the sample. The technique is applicable to a wide size range from sub-nanometer up to a few microns. In order to obtain a meaningful result, a minimum scattering signal strength must be present. Malvern’s Zetasizer Nano software contains a calculator to help estimate the required minimum concentration.
If anyone else has …

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Binding affinity and Kd, Differential Scanning Calorimetry, Dynamic Light Scattering, Français, Gel permeation chromatography, Image Analysis, Isothermal Titration Calorimetry, Laser Diffraction, Meet the Experts, Molecular size, Nanoparticle Tracking Analysis, Particle concentration, Particle size, Rheology, Rheometry, Size Exclusion Chromatography, Static Light Scattering, Zeta potential »

[21 Feb 2017 | ]

Afin de vous apporter un support concret pour utiliser votre système de façon optimale, finaliser vos bonnes pratiques de laboratoire et répondre à vos besoins spécifiques, Malvern organise des formations sur site ou en sessions dans nos 3 agences françaises (Orsay, Lyon et Toulouse).
Voici les dates de toutes les sessions programmées pour 2017 :
CARACTERISATION DES PARTICULES PAR DIFFRACTION LASER
Objectifs : Perfectionner les méthodes de caractérisation des particules en voie sèche et liquide, définir les bonnes pratiques de laboratoire et s’adapter aux besoins spécifiques de chaque installation. Comprendre le principe physique …

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Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[15 Sep 2016 | ]

Nanoparticle Tracking Analysis or Dynamic Light Scattering ?
After a recent joint NanoSight and Zetasizer demonstration, I received this set of questions that helps illustrate the core of the issue of these two techniques:

Since the Nanosight gives me particle concentration, it’s somewhat straightforward to calculate what % of my Nanoparticles are aggregated. Can I calculate % aggregated particles in the DLS as well?  Or does the varying amount of intensity, and the protein+nanoparticle mixture in the sample confound that calculation?

If I wanted to measure non-spherical particles (for example nanorods), which system …

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Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle size, Protein Aggregation, Tech Talk »

[26 May 2016 | ]

We recently published our application note, “Adeno-Associated Virus titer and aggregation characterization“, which discusses a new NanoSight method that allows the visualization and therefore analysis of viruses smaller than what was previously possible to measure.  This represents an exciting extension of the Nanoparticle Tracking Analysis (NTA) technique in response to market demand for tools for better characterization of small virus types.
 
Adeno-Associated Virus (AAV) is commonly used in the gene therapy field as a delivery vector. Tools to characterize both the concentration and purity or aggregation of the samples are limited because the primary virus size is …

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Dynamic Light Scattering, Particle concentration, Particle size, Tech Talk, Zetasizer »

[3 Mar 2016 | ]

For fluorescent samples (like quantum dots) the Zetasizer has an option that can minimize the effect of (incoherent and thus undesirable for dynamic light scattering) fluorescent light: the narrow band filter eliminates most light that is not very close to the laser wavelength.
Narrow band filter – how it works and specifications
The narrow band filter is an optical component made out of glass with a special coating to reflect light outside the designated wavelength range. Only a select range (in the illustration, the red light) can be transmitted.
In the Zetasizer, the …

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