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Dynamic Light Scattering, Kinexus, Laser Diffraction, Mastersizer, Nanoparticle Tracking Analysis, Nanosight, Rheometry, Size Exclusion Chromatography, Tech Talk, X-Ray Diffraction (XRD), Zetasizer »

[5 Dec 2017 | ]

It was my great pleasure to present as part of the Worcestershire Innovation Network at their WINN Wednesday event on the 22nd of November, showcasing the best in innovation across Worcestershire.
This included a local company which makes artificial kneecaps and hips, an insight into the building of genomics studies of patients with rare diseases and cancers, an update on the appeal to get a state-of-the-art surgical robot for removal of prostate tumors (reducing discomfort, scarring and hospital stay post-operation). Jess Antley, head of WINN, also gave an update on a new shared space/collaboration …

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Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Tech Talk »

[23 Nov 2017 | ]

Results of a multi-round interlaboratory comparison (ILC) of NanoSight instruments were recently published1, setting a benchmark for repeatability and reproducibility for the Nanoparticle Tracking Analysis (NTA) technique. The limits of measurement were shown to extend beyond the 30 nm –600 nm range (with all NanoSight models), with percentage coefficients of variation (% CV), calculated as sub 5% for monodisperse samples. The process further highlighted how much variability or error might be introduced if inappropriate instrument settings or analytical procedures are used.
As a relatively new technique, there remained questions about the …

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Differential Scanning Calorimetry, Dynamic Light Scattering, MicroCal, Molecular size, Molecular structure, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[16 Nov 2017 | ]

Measuring aggregation temperature using the Zetasizer

In dynamic light scattering (DLS), the hydrodynamic size and size distribution of particles in solution can be obtained.  It may be of interest to examine this measurement as a function of time and temperature, or as a ‘trend’, as it is called in the Malvern Zetasizer software.  For biological protein samples, this temperature trend is particularly interesting: although at low temperatures a protein may be stable and show repeatable size (and scattering intensity) measurements, typically at some elevated temperature (Tagg), protein molecules will show a tendency …

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ASD, Ground truthing, Near-InfraRed spectroscopy (NIR), Remote sensing, Tech Talk »

[14 Nov 2017 | ]

This November, the ASD webinar focuses on hyperspectral imaging, near infrared spectroscopy (NIRS), and multivariate calibration models (i.e. chemometrics), featuring ASD’s FieldSpec® 4 Hi-Res NG Spectroradiometer.

In this webinar, you will learn:

How the combination of hyperspectral imaging, near-infrared spectroscopy (NIRS), and multivariate calibration algorithms (i.e. chemometrics), enables quantitative mapping of properties of the Earth’s surface
The data that ground-based reflectance measurements of materials can provide for remote sensing applications
How to apply quantitative chemometric calibrations to hyperspectral image data
Examples of successful applications

November’s ASD webinar speaker is Dr. Brian Curtiss; Brian is one of …

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ASD, Tech Talk »

[17 Oct 2017 | ]

ASD has long been the gold standard in field spectroradiometers. ASD pioneered the science of field spectroscopy over 25 years ago and continues to lead the industry with the world’s most trusted field-portable UV/Vis/NIR/SWIR spectroradiometers covering the full solar reflected spectrum.
ASD’s spectroradiometers and spectrometers are portable and deliver fast and accurate spectral measurements. These spectral measurements act as fingerprints, providing important data that leads to solutions in a variety of applications, such as:

Atmospheric research
Forestry, ecology and plant physiology studies
Light energy measurements
Spectroradiometric calibrations
Ingredient testing for dietary supplements and natural products
Lipid, protein …

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Dynamic Light Scattering, Particle size, Tech Talk, Zetasizer »

[26 Sep 2017 | ]

Is there a maximum viscosity for DLS measurements?
 
The Malvern Zetasizer can determine particle size by dynamic light scattering (DLS). In this technique, the intensity fluctuations are analyzed to find the corresponding diffusion coefficient that led to the fluctuations. The translational diffusion coefficient Dt of a particle is inversely related to its size and the viscosity of the dispersant (or more accurately the hydrodynamic radius rH and viscosity η).

This relationship can be used to predict detectability limits of the technique in general, and this is how we explore the idea of …

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Archimedes, Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Image Analysis, Label-free analysis, MicroCal, Microcalorimetry, Morphologi G3-ID, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Rheology, Size Exclusion Chromatography, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Viscotek, Zetasizer »

[21 Sep 2017 | ]

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was a range of scientists and researchers from all over the world, with one common subject in mind: Protein Stability.

One of the highlights of this conference was the pre-conference workshop. This was a free workshop for all attendees of the conference, which was sponsored by Malvern Panalytical. The workshop included a range …

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Binding affinity and Kd, Isothermal Titration Calorimetry, MicroCal, Tech Talk »

[12 Sep 2017 | ]

Binding Affinity
 
Understanding binding affinity is key to appreciation of the intermolecular interactions driving biological processes, structural biology, and structure-function relationships. It is also measured as part of the drug discovery process to help design drugs that bind their targets selectively and specifically.
We recently launched a new short video showcasing the MicroCal PEAQ-ITC and how it is an essential piece to any biological interaction analysis toolkit for measuring Binding affinity and stoichiometry.

The MicroCal PEAQ-ITC offers key benefits over other ITC systems including:
PEAQ-Expert: New easy-to-use software to guide your way includes built-in …

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Electrophoretic light scattering, Tech Talk, Zeta potential, Zetasizer »

[5 Sep 2017 | ]

Zetasizer: What is the zeta potential deviation?
 
The Malvern Zetasizer software report for zeta potential shows a parameter that is called Zeta deviation. This parameter is shown directly below the mean Zeta potential.

The mean zeta potential is derived from the fast field reversal (FFR) phase analysis light scattering (PALS) technique.
The fast field reversal provides a single number from the analysis. This single number is the mean zeta potential. The slow field reversal [also known as SFR] can be used to determine the zeta distribution, a distribution of different charge values from …

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Morphologi G3-ID, Particle shape, Raman spectroscopy, Tech Talk »

[31 Aug 2017 | ]

GEN covers the role of MDRS in reducing the need for clinical bioequivalence endpoint studies
 
Morphologically-Directed Raman Spectroscopy (MDRS) combines the capabilities of automated imaging with Raman spectroscopy in a single platform, creating a powerful analytical tool that spans multiple industries. It exhibits particular value in both innovator and generic pharmaceutical development, as detailed particle characterization enables a thorough understanding of individual materials and how they impact the final product – as demonstrated by a recent Malvern Panalytical article, featured on the GEN website.
 
Raman spectroscopy and MDRS enable determination of the …

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Laser Diffraction, Mastersizer, Particle size, Tech Talk »

[1 Aug 2017 | ]

Just when I thought I’d seen everything that could contaminate the background, a user sent me this picture! The Musca Domestica (common housefly) was attracted by certain aromatics used in testing and seems to have entered the measurement cell through an empty Hydro EV over a weekend. Unfortunately, it became wedged within the measurement cell and did not survive the experience.

The most important message from the Mastersizer training courses I give is to make sure you have a good background before you start. Laser diffraction is a background subtracted technique: …

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Dynamic Light Scattering, Tech Talk, Zetasizer »

[11 Jul 2017 | ]

A webinar entitled “DLS in 30 Minutes” was given in June which attracted a very large audience. The presentation introduced dynamic light scattering and covered the principles of the technique, a discussion of how the measurement is performed, how the data is analyzed and how the results obtained should be interpreted.
We were inundated with various questions during the webinar and we only had time to answer a few on the day. The remainder of questions were answered via e-mail but we thought it would be good to share some of …

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Laser Diffraction, Mastersizer, Meet the Experts, Particle size, Tech Talk »

[13 Jun 2017 | ]

I recently presented a webinar exploring the practical aspects of sieving method – the advantages and disadvantages.  I compared the method to other techniques such as laser diffraction indicating the increased wealth of information that can be obtained with these more modern instrumental techniques and the common pitfalls and assumptions of the screening technique.
More than 800 of you registered for the webinar and I received a large numbers of interesting questions which I have been working steadily through. I thought it would be a good idea to share the questions and answers via the blog, so here they …

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Kinexus, Rheology, Tech Talk »

[8 Jun 2017 | ]

How to best measure a viscosity flow curve?
 
Choosing the right measuring geometry and preventing measurement artifacts
There are several ways to obtain viscosities at different shear rates using a rotational rheometer. For instance, we could gently ramp through the different speeds starting at the lowest and ending at the highest (good for a quick measurement where time is of the essence).  Alternatively, we could step through the required shear rates as discussed later, while waiting for a steady state viscosity at each step.  This is known as generating an equilibrium flow …

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Meet the Experts, Particle size, Tech Talk »

[30 May 2017 | ]

On April 26th, 2017, I had the great pleasure of being invited to talk as part of the very first Malvern TEDx evening, held at the Coachhouse Theatre in Great Malvern.  It was a really enjoyable event, and the talks are now on YouTube for you all to view!

I gave a top-level overview of how so many of the products and devices we consume or encounter on a daily basis are specifically designed by chemists and other scientists, so that they work the way we need or want them to. From …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, MicroCal, Microcalorimetry, Molecular weight, Tech Talk »

[23 May 2017 | ]

A professional and personal view
If your newsfeed from scientific and mainstream media sources is anything like mine, you have seen numerous articles and blogs about the ‘reproducibility crisis’ in the life sciences.
Well-established cornerstones of scientific inquiry and research are the publication of findings in peer-reviewed journals, along with the opportunity for others to reproduce and verify published data.  This advancement and consolidation of knowledge leads to further understanding on how biological systems work, and provides the basis for the discovery and development of new drugs which benefit all of us.
However, there …

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Dynamic Light Scattering, Meet the Experts, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Zetasizer »

[18 May 2017 | ]

Matt McGann and I will be representing Malvern at the upcoming annual UD Biophysical Characterization Workshop, sponsored by CBST (Center for Biomanufacturing Science & Technology), BITC (Biomolecular Interaction Technologies Center), and the University of Delaware, on June 6, 2017, hosted by Professor Christopher Roberts.
This year’s workshop will consist of three days of short courses and small-group demonstrations of the principles and commercial technologies for characterization of protein solutions and related colloidal systems, focusing specifically on static and dynamic light scattering, electrophoresis (DLS, ELS), and Taylor Dispersion Analysis (TDA).
Matt has been …

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Image Analysis, Laser Diffraction, Mastersizer, Morphologi G3, Tech Talk »

[13 Apr 2017 | ]

The prevalence of chocolate eggs and chocolate bunnies in the supermarkets here in the UK can only signal one thing: Easter is upon us.  With the emergence of daffodils, buds appearing on trees, lambs frolicking in the fields and warmth seeping back into the northern hemisphere, the cold winter months are over.  Of course, it is not just in the western world that the arrival of spring, new life and new beginnings are celebrated.
In Japan, a country I had the pleasure of living in for a year and a half …

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Customer experience, Insitec, Kinexus, Mastersizer, Morphologi G3-ID, Tech Talk »

[30 Mar 2017 | ]

The recently announced merger of Malvern Instruments and PANalytical could not be better timed from the Cement industry’s perspective. Both companies are market leaders in their respective technology fields – Malvern in particle characterization and PANalytical in elemental and mineralogical analysis – and both having strong teams of application experts with a lot of experience in cement production and characterization.
The synergy between the teams and product offerings was described in a recent webinar by Harald van Weeren of PANalytical and myself. The live event was attended by over one hundred participants and …

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Dynamic Light Scattering, Molecular size, Particle concentration, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[7 Mar 2017 | ]

How can I use DLS for sample xyz?
What sample concentration / refractive index is needed for reliable DLS size measurements?

Has it been done before?
In Dynamic Light Scattering (DLS) intensity fluctuations in the sample are analyzed to obtain size information on the sample. The technique is applicable to a wide size range from sub-nanometer up to a few microns. In order to obtain a meaningful result, a minimum scattering signal strength must be present. Malvern’s Zetasizer Nano software contains a calculator to help estimate the required minimum concentration.
If anyone else has …

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