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MicroCal microcalorimeters are powerful, analytical tools used to study biological systems. These include interactions between molecules as well as changes in conformation such as protein unfolding. The technique is very convenient requiring only minimal assay development and no labelling. The range includes both Differential Scanning and Isothermal Titration calorimetry.

Binding affinity and Kd, Isothermal Titration Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[12 Oct 2017 | ]

Isothermal Titration Calorimetry
Molecular recognition is key in all biological processes and cellular responses. It is critical to characterize the structural and thermodynamic forces behind the recognition and specificity of macromolecular interactions in order to understand the molecular events in a cell. Several biophysical techniques (including fluorescence, circular dichroism, Fourier transform infrared spectroscopy, mass spectroscopy, surface plasmon resonance, and microscale thermophoresis) are used to characterize non-covalent molecular interactions.
Isothermal titration calorimetry (ITC) has become a popular and versatile bioanalytical method for the direct measurement of binding affinity between interacting molecules.  ITC instruments were …

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Archimedes, Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Image Analysis, Label-free analysis, MicroCal, Microcalorimetry, Morphologi G3-ID, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Protein higher order structure, Raman spectroscopy, Resonant mass measurement, Rheological and Viscosity, Size Exclusion Chromatography, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Viscotek, Zetasizer »

[21 Sep 2017 | ]

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was a range of scientists and researchers from all over the world, with one common subject in mind: Protein Stability.

One of the highlights of this conference was the pre-conference workshop. This was a free workshop for all attendees of the conference, which was sponsored by Malvern Panalytical. The workshop included a range …

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Binding affinity and Kd, Isothermal Titration Calorimetry, MicroCal, Tech Talk »

[12 Sep 2017 | ]

Binding Affinity
 
Understanding binding affinity is key to appreciation of the intermolecular interactions driving biological processes, structural biology, and structure-function relationships. It is also measured as part of the drug discovery process to help design drugs that bind their targets selectively and specifically.
We recently launched a new short video showcasing the MicroCal PEAQ-ITC and how it is an essential piece to any biological interaction analysis toolkit for measuring Binding affinity and stoichiometry.

The MicroCal PEAQ-ITC offers key benefits over other ITC systems including:
PEAQ-Expert: New easy-to-use software to guide your way includes built-in …

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Differential Scanning Calorimetry, Dynamic Light Scattering, Meet the Experts, MicroCal, Molecular size, Molecular weight, OMNISEC, Particle concentration, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Zetasizer »

[18 Jul 2017 | ]

Monoclonal Antibodies – or mAbs for short

Since many of these mAbs require refrigeration we had used dynamic light scattering (with a Zetasizer) to show the formation of aggregates after freeze-thaw cycles (for details see application note “Effect of storage conditions on Immunoglobulin“). These could be seen both in the increased polydispersity index (PDI) and the appearance of a large-size aggregate peak in the intensity size distribution as shown to the right. The area under the peak gives an indication of the presence of the large aggregates but is not suited for …

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Differential Scanning Calorimetry, Meet the Experts, MicroCal, Microcalorimetry, Protein higher order structure »

[4 Jul 2017 | ]

Protein Antigens and DSC

This blog highlights two recent articles that focus on the use of a MicroCal Differential Scanning Calorimetry (DSC) system.
Differential Scanning Calorimetry – A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
I.B. Duruwoju, K.M. Bhandal, J. Hu, B. Carpick, and M. Kirkitadze
Journal of Visualized Experiments (JoVE), Issue 121, e55262, doi:10.3791/55262 (2017)
The authors (from Sanofi Pasteur in Toronto, Canada), provide a detailed protocol to perform Differential Scanning Calorimetry experiments on an automated DSC, such as a MicroCal VP-Capillary DSC system from Malvern Instruments.  This piece is a JoVE …

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Archimedes, Customer experience, Differential Scanning Calorimetry, Gel permeation chromatography, Image Analysis, MicroCal, Morphologi G3-ID, Nanoparticle Tracking Analysis, Nanosight, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Viscotek »

[1 Jun 2017 | ]

Our key driver at Malvern Instruments is to create technologies that make a difference or solve a problem, and working hand-in-hand with our customers enables us to make sure that’s exactly what’s happening, out in the field.  We place a high value on our collaborations and partnerships with customers from a wide range of industries and organizations across the globe and love to see how our systems are being used in real-world scenarios.  This gives us vital feedback on what we’re doing well, and also any tweaks we need to make in order to optimize …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, MicroCal, Microcalorimetry, Molecular weight, Tech Talk »

[23 May 2017 | ]

A professional and personal view
If your newsfeed from scientific and mainstream media sources is anything like mine, you have seen numerous articles and blogs about the ‘reproducibility crisis’ in the life sciences.
Well-established cornerstones of scientific inquiry and research are the publication of findings in peer-reviewed journals, along with the opportunity for others to reproduce and verify published data.  This advancement and consolidation of knowledge leads to further understanding on how biological systems work, and provides the basis for the discovery and development of new drugs which benefit all of us.
However, there …

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Archimedes, Chemical identification, Fluorescence Detection, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry, Molecular size, Molecular structure, Molecular weight, Morphologi G3-ID, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Protein higher order structure, Protein Mobility, Viscosizer TD, Zeta potential, Zetasizer »

[16 May 2017 | ]

With Pre-Conference Workshop on Biophysical Characterization and Particle Analysis
 

Date: July 17th – 20th.
We at Malvern know that protein stability is important in numerous disciplines, ranging from basic and medical biochemistry to pharmaceutical sciences. However, it is rare that researchers from all the relevant areas can join to discuss the critical issues in the field. The 2017 Colorado Protein Stability Conference provides a unique forum for this exchange of information. The topics to be covered include protein folding, dynamics and thermodynamic stability; amyloidosis and protein aggregation; and stability, formulation, analysis and processing …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[11 May 2017 | ]

In September 2016 Malvern Instruments co-organized a European MicroCal meeting with Institut Pasteur and ARBRE European molecular biophysics network. The meeting was very successful and sparked animated discussions during scientific presentations, panel and poster sessions and interactive workshops.  A key focus was on best practice for experimental design and data analysis to ensure high-quality data and informed decisions that help drive scientific projects forward.
In two days, together with more than 120 participants from 19 countries, we covered a wide range of topics, from interaction and kinetic analysis with ITC to …

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Archimedes, MicroCal, Nanosight, OMNISEC, Viscosizer TD »

[27 Apr 2017 | ]

The LABVOLUTION with Life Sciences Event BIOTECHNICA will take place from 16-18 May 2017 in Hanover. This event is the European trade fair for innovative laboratory equipment and for the optimization of laboratory workflows, with a focus on biotech solutions.
Visit us in Hall 20 Stand E59 and discover how to profile and stabilize your proteins and other biomolecules.
Please contact us and get your FREE ENTRY TICKETS!

Discover new technologies for the biophysical characterization of your biomolecules – measuring systems and technologies:

MicroCal ITC / DSC – The gold standards for interaction, stability and biocomparability analysis
Zetasizer …

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Archimedes, Malvern Panalytical Events, Meet the Experts, MicroCal, OMNISEC, Viscosizer TD »

[14 Mar 2017 | ]

Malvern Instruments is known for particle sizing technologies. Did you know that many of our instruments are used to characterize proteins, nucleic acids, liposomes, exosomes, and other biomolecules?
Our instruments are used in academic and government research labs, as well as pharmaceutical and biotech companies.
In North America, we have a team of field application scientists and sales consultants who are focused on biomolecule characterization using microcalorimetry, light scattering, Taylor dispersion analysis, nanoparticle tracking analysis, resonant mass measurement, and size-exclusion chromatography.
In the upcoming months, Malvern representatives are attending the scientific conferences listed …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry, Protein higher order structure »

[1 Mar 2017 | ]

The NEW MicroCal PEAQ-DSC – the gold standard for thermal stability determination
Differential scanning calorimetry (DSC) is an established biophysical tool for protein stability characterization during drug development. DSC data are used to advance the most stable and ‘developable’ proteins into the pipeline, and optimize the process and formulation conditions which will maintain protein stability during manufacturing and storage. DSC data are also important in higher order structure (HOS) protein characterization, lot-to-lot comparability, and characterization of biosimilar drugs compared to innovators.
Our previous technology, the MicroCal VP-Capillary DSC Automated system, is a …

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Differential Scanning Calorimetry, Malvern Panalytical Events, MicroCal, Tech Talk »

[14 Feb 2017 | ]

My introduction to Differential Scanning Calorimetry (DSC) was a postdoctoral fellow in the late 1980s.  I was producing mutant forms of the enzyme thymidylate synthase, and I needed to characterize the effects amino acid changes had on the overall stability of the protein.  Using a DSC instrument at our facility, I compared the thermal stability of the different mutants to wild-type protein by ranking the thermal transition temperature (TM). As I recall, I needed about 2 mg -4 mg protein per DSC experiment, which was a lot of protein at …

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Français, Malvern Panalytical Events, MicroCal, OMNISEC, Viscosizer TD »

[9 Feb 2017 | ]

 
Nous serons présents sur le Salon Forum Labo du 28 au 30 Mars 2017 sur le stand D54 E55 face au stand de PANalytical.
Cette proximité permettra de démontrer la complémentarité de nos gammes de mesure puisque Malvern et PANalytical ont fusionné leur activité depuis le 1er Janvier 2017.
Lors du salon Forum Labo 2017, vous pourrez découvrir :

Le nouveau microcalorimètre différentiel à balayage MicroCal PEAQ-DSC permettant le criblage d’un grand nombre d’échantillons à faible concentration, ce qui minimise la consommation d’échantillons précieux et le temps d’intervention des utilisateurs.  La DSC mesure …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry, Protein higher order structure »

[2 Feb 2017 | ]

We are proud to announce Malvern Instruments’s Gold Sponsorship of Global Engage’s Biosimilars and Biologics Congress 2017.  This two-day event will be taking place on Monday 6th and Tuesday 7th March 2017, at the Leonardo Royal Hotel Berlin Alexanderplatz.
This meeting will be attended by industry, regulatory and academic experts from all areas of protein, antibody and biosimilar research and will comprise three topic streams: Innovations in Antibody-based Therapeutics; Expression, Purification and Characterization (with a particular focus on product stability and half-life); Biosimilars.
Natalia Markova, Principal Scientist, Malvern MicroCal, will be discussing …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry, Protein higher order structure »

[31 Jan 2017 | ]

DSC for the regulated environment
 

 
Advancing biotherapeutic molecules along the drug development pipeline is a complex, time-consuming and expensive process. Good stability is key to a candidate’s progression, and must be assessed and reviewed throughout development – from candidate selection, through formulation development, all the way to batch-to-batch comparability or biosimilarity assessment….and beyond! Objective, reliable stability data is needed for confident decision-making about which drug products and processes to move through development into commercialization.
Differential Scanning Calorimetry (DSC) is a biophysical tool for protein stability characterization. The MicroCal VP-Capillary DSC system, launched in 2002, …

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Isothermal Titration Calorimetry, MicroCal, Microcalorimetry »

[13 Dec 2016 | ]

Ah, the holidays are here! This is the time of year when we enjoy special ‘once a year’ holiday meals and treats with family and friends. There are the succulent cuts of meat, marbled with fat…..perhaps you have a holiday goose or duck. There are the vegetables and pastas coated with butter, cheese, or cream sauces. There are the rich, buttery desserts. Let’s not forget the candy, chips, eggnog, and almost every delicious, decadent item we eat or drink. We all have our favorite holiday dishes, which are likely high …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Panalytical Events, MicroCal »

[9 Nov 2016 | ]

I always enjoy meeting our Malvern customers whenever I travel to different sites and events. I have been assisting customers with MicroCal ITC (Isothermal Titration Calorimetry) and DSC (Differential Scanning Calorimetry) instruments for over 15 years, and there are customers I have known since 2001. Every time I think I “know it all” I always learn something new from our customers, such as a unique sample analyzed using our instruments, or a “trick” for sample preparation or data analysis.
Malvern Instruments recently hosted two MicroCal “User Days” in San Diego, CA …

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Isothermal Titration Calorimetry, Malvern Panalytical Events, MicroCal »

[25 Oct 2016 | ]

Thermodynamics – Recap of the 30th Annual Gibbs Conference on Biothermodynamics 
ΔG = ΔH – TΔS
 
Many scientists will recognize this equation, relating the enthalpy and entropy change of a reaction to ΔG or “Gibbs energy.”  But do you know anything about the man behind this expression of energy?
Josiah Willard Gibbs (1839 –1903), spent most of his life in New Haven, Connecticut. His work on the applications of thermodynamics was instrumental in transforming physical chemistry into a rigorous deductive science. Together with James Clerk Maxwell and Ludwig Boltzmann, he created statistical mechanics (a term that Gibbs coined), explaining the laws of thermodynamics as consequences of the …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Panalytical Events, Meet the Experts, MicroCal »

[5 Oct 2016 | ]

Onto Day 2 of the meeting in the beautiful sunshine of Paris, with another agenda full to brimming with a great set of inspiring presentations.
The first session of the morning was chaired by Malvern’s Aymeric Audfray, and was entitled: “Developments in ITC applications into challenging areas”. The session was kicked off by Anne Imberty, who spoke about “ITC for structural glycosciences tools”.  This talk was followed by Laurent Bouteiller from Pierre and Marie Curie University who delivered a presentation on using “ITC as a tool for the characterization of self-assembled systems in organic solvents”.
Interaction with the …

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