The Malvern NanoSight range of instruments utilizes Nanoparticle Tracking Analysis to characterize nanoparticles from 10nm -2000nm in solution. Each particle is individually but simultaneously analyzed by direct observation and measurement of diffusion events.
Dynamic Light Scattering, Français, Laser Diffraction, Malvern Events, Meet the Experts, Nanosight, Particle size, Static Light Scattering, Zetasizer »
Les 20èmes rencontres organisées à Rennes auront lieu du 28 au 30 novembre avec la collaboration l’Institut des Sciences Chimiques de Rennes et la plateforme ScanMat. La directrice de cette plateforme, Maryline Guilloux-Viry, nous fera l’honneur d’accueillir les participants et de présenter les moyens et compétences disponibles au sein de la fédération des 5 plateformes constituant ScanMat (Microscopies électroniques, Analyses structurales et élémentaire, Synthèse et mise en forme, Spectroscopies et Microspectroscopies optiques).
Les nanoparticules seront au cœur de ces rencontres avec une évocation des nouvelles réglementations de REACH qui seront en vigueur …
Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle size, Protein Aggregation, Tech Talk, Zetasizer »
Nanoparticle Tracking Analysis or Dynamic Light Scattering ?
After a recent joint NanoSight and Zetasizer demonstration, I received this set of questions that helps illustrate the core of the issue of these two techniques:
Since the Nanosight gives me particle concentration, it’s somewhat straightforward to calculate what % of my Nanoparticles are aggregated. Can I calculate % aggregated particles in the DLS as well? Or does the varying amount of intensity, and the protein+nanoparticle mixture in the sample confound that calculation?
If I wanted to measure non-spherical particles (for example nanorods), which system …Read the full story »
Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle size, Protein Aggregation, Tech Talk »
We recently published our application note, “Adeno-Associated Virus titer and aggregation characterization“, which discusses a new NanoSight method that allows the visualization and therefore analysis of viruses smaller than what was previously possible to measure. This represents an exciting extension of the Nanoparticle Tracking Analysis (NTA) technique in response to market demand for tools for better characterization of small virus types.
Adeno-Associated Virus (AAV) is commonly used in the gene therapy field as a delivery vector. Tools to characterize both the concentration and purity or aggregation of the samples are limited because the primary virus size is …
Malvern Events, MicroCal, Nanosight, Zetasizer »
From nanoemulsions, nanosuspensions, vaccines and adjuvants to drug formulation and release development, this year’s American Association of Pharmaceutical Scientists (AAPS) National Biotech Conference has the strapline: “Advancing health through innovation in biotherapeutics“.
Come visit us at booth #400 at the Hynes Convention Center in Boston, Massachusetts, to find out what Malvern is doing to innovate in the analytical biotech sector on May 16 – 18, 2016.
Poster Session highlights
There are two poster sessions at the meeting (Monday and Tuesday, Hynes Convention Center – Hall D), and quite a few posters, with presenters from Mercer University, …
Meet the Experts, MicroCal, Nanosight, Viscosizer TD, Zetasizer »
We are proud to be sponsoring the 9th Annual Proteins and Antibodies Congress, to be held at the Novotel London West, UK on 18th and 19th April 2016. Malvern MicroCal’s Principal Scientist, Dr. Natalia Markova, will be chairing the Protein Expression and Characterization Stream on the morning of Day 1, and will go on to deliver her own presentation on high resolution profiling and optimization of antibody stability through size distribution and conformational stability studies at 14.50 that same afternoon.
The event is co-located with two other congresses: the 3rd Annual Peptides …
Electrophoretic light scattering, Nanosight, Protein Mobility, Tech Talk, Zeta potential, Zetasizer »
How to perform zeta potential measurement with the diffusion barrier method?
The diffusion barrier method is particularly suitable for low sample volumes and high ionic strength buffers. A “plug” of the sample is carefully injected into the folded capillary cells which is filled with just the buffer (The buffer has to be the same buffer that the protein is prepared in, same conductivity, same pH, same additives, in order to match the sample and the diffusion barrier as closely as possible). In this setup, primarily the zeta potential is of interest, however …Read the full story »
Molecular weight, Nanosight »
Higher Order Structure Consortium for biopharmaceutical proteins
Case studies on technical decision making with biophysical data
During the development of proteins for use as biopharmaceuticals, the primary structure (amino acid sequence) is important in defining protein activity.
Due to the complex nature of protein drugs, compared to small molecule drugs, biophysical techniques are important tools in the characterization of the protein’s higher-order structure (HOS) to understand the protein’s stability, folding, structure, and functional activity. HOS is characterized variety of biophysical methods including:
Mass spectrometry (MS)
Circular dichroism (CD)
Fourier transform infrared spectroscopy (FTIR)
Nuclear magnetic …
Customer experience, Malvern Events, Nanosight, Viscotek, Zetasizer »
San Francisco, CA. If you are in the Northern California Bay area then this might be of interest. We are exhibiting at the AAPS National Biotech meeting next week, and complimentary registrations are available for the exhibit at no cost to you.
Visit us at booth #412 in the San Francisco Marriott Marquis – Yerba Buena Ballroom and talk with a Malvern expert to find out more about:
Ultimate sensitivity in advanced chromatography detection, brand new OMNISEC
Combining the easiest-to-use SEC-MALS with Dynamic Light Scattering (DLS)
Finding structural information with Raman and the new …
Here at Malvern, the NanoSight team is preparing for the International Society of Extracellular Vesicles (ISEV) meeting this week in Washington, D.C. and can’t wait to attend what will be the biggest extracellular vesicle conference to date. The widespread use of NanoSight systems in exosome and microvesicle research makes this one of our best chances to interact closely with users in this application space. We will have three technical specialists on site to field any questions you have regarding the use of the Nanoparticle Tracking Analysis (NTA) technique for vesicle …Read the full story »
Malvern Events, Meet the Experts, MicroCal, Nanosight, Zetasizer »
We are offering four very exciting life science seminars in the Netherlands this month and the best part is that there is no charge to attend. There are several options depending on your specific area of interest, covering advanced training in MicroCal DSC/ITC, NanoSight Nanoparticle Tracking Analysis, and the Zetasizer Nano range. There is also a general afternoon session introducing each of the characterization solutions for Life Sciences from Malvern Instruments, including new technology you may not have seen before – the Zetasizer Helix combines DLS & Raman Spectroscopy and the new Viscosizer measures …Read the full story »
Dynamic Light Scattering, Malvern Events, Meet the Experts, Nanoparticle Tracking Analysis, Nanosight, Particle size, Zeta potential, Zetasizer »
Malvern Instruments is heading to Pittcon 2015 this weekend in New Orleans, and while a lot of our time and energy is focused on making sure people get a great experience when visiting our booth (#3,545) or attending our short courses, we’re always keen to see what else is going on in the great conference and networking program. While preparing material for next week, I took a look at the conference program and was excited to see the description of the Wallace H. Coulter lecture that Naomi J. Halas, who …Read the full story »
Back in 2008 we published an article on dynamic light scattering (DLS) from sucrose solutions. This was quite a project, as we had previously seen the effect of ‘solvent peaks’ yet never thoroughly investigated these. In the article titled “Measuring sub nanometre sizes using dynamic light scattering” we showed that a peak below 1 nm was indeed due to the Brownian motion from sucrose molecules in solution, and then backed up the claim with supporting evidence. What we had stumbled across but were not quite able to explain was a recurring peak …Read the full story »
Malvern Events, Mastersizer, Nanosight, Particle size, Tech Talk, Zetasizer »
This bad pun is the title of my next webinar scheduled for February 18th. This title has also been used in a short book of poems by Mark Begley (of which I have copy 33/35…).
I guess you couldn’t ask for a better mix of art, science, myth, intrigue and a murder or three – ideal Valentines viewing. Indeed “it would be difficult to find a liquor with a more sordid past than absinthe.”
A full 2 years before the forerunner of the FDA in the USA banned cocaine and heroin (in …
Archimedes - Particle Metrology System, Insitec, Laser Diffraction, Malvern Events, Mastersizer, Morphologi G3-ID, Nanosight, Spraytec, Tech Talk, Zetasizer »
As a confirmed space-geek ever since Halley’s Comet in 1986, lasers, or perhaps I should say laser guns in their various forms, have always been a part of my life. I’m not sure if I first met lasers in Star Wars or being fired at Doctor Who by a Dalek. But I do know they made a big impression on me. The lasers of sci-fi are flashy affairs, complete with awesome graphics, sound effects and implausible physics. Perhaps the most iconic being the lightsaber of Luke Skywalker. So I was …Read the full story »
Dynamic Light Scattering, Electrophoretic light scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Tech Talk, Zeta potential, Zetasizer »
The Consumer Electronics Show (CES) took over Las Vegas once again last week, and this time, quantum dots are the hottest thing happening in TV technology. At CES 2015 LG revealed a 4K quantum dot TV, the UF9400, quickly followed by Samsung‘s own line of Ultra HD TVs featuring quantum dots. Sony was one of the first tech companies off the mark, including quantum dot technology in some of its high end TVs back in 2013.
Quantum dots are uniquely highly scattering nanoparticles that can be used for image enhancement. These light-emitting semiconductor nanocrystals …
Dynamic Light Scattering, Meet the Experts, Molecular size, Nanosight, Particle size, Zetasizer »
Tips & Tricks for Nanoparticle Characterization
A few days ago we hosted a live webinar titled “Nanoparticle Size Characterization: Tips & Tricks with the Zetasizer Nano.” This seems to have been a topic of interest for many of you, since – with apologies – I did not get to answer all the questions coming in at the end of the presentation. So here is my follow-on to the event.
To recap this abstract was posted before the event: Nanoparticles are defined by their size. In this presentation different sizing techniques are …
Archimedes - Particle Metrology System, Dynamic Light Scattering, Molecular weight, Nanoparticle Tracking Analysis, Nanosight, Particle size, Resonant mass measurement, Tech Talk, Zeta potential, Zetasizer »
Having spent more than twenty years in the instrumentation arena and worked with a variety of different characterization techniques in lots of different application spaces, I have sometimes struggled to explain to my family what it is that I do. Particularly to my two young children who have never quite got the intricacies of nanomaterial characterization despite my attempts to stress the importance by referring to everyday products like computers and iPods that they take for granted.
So it was within this context that I found myself generating a presentation on the important and evolving sector of …
We’re headed to the American Association of Pharmaceutical Scientists (AAPS) Annual Meeting & Exposition this week for what promises to be a really interesting event in (hopefully) sunny San Diego. You can find us at booth 330, where we’ll have a whole load of new & innovative tech to help you with pharmaceutical and biopharmaceutical discovery, development & manufacture.
Malvern’s portfolio of instruments for pharmaceutical scientists has expanded significantly in the last year. The acquisition of MicroCal’s microcalorimetry range has extended our capabilities to include drug discovery applications for both small and …
Archimedes - Particle Metrology System, Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Resonant mass measurement, Tech Talk, Zetasizer »
PhD student Polly came into the lab on Monday morning to find that her purified protein had started to aggregate after a weekend in the fridge. The profile she got from her Dynamic Light Scattering (DLS) measurement clearly showed two peaks, with the second in the micron range. Her supervisor suggested she tried Nanoparticle Tracking Analysis (NTA) to get more information on the different sized aggregate species in her sample. This showed that there were aggregates of many different sizes present. The question was, what should she do next?
Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Tech Talk, Zetasizer »
PhD student Polly came into the lab on Monday morning to find that her purified protein had started to aggregate after a weekend in the fridge. The profile she got from her Dynamic Light Scattering (DLS) measurement clearly showed two peaks, with the second in the micron range. Her supervisor suggested she tried Nanoparticle Tracking Analysis (NTA) to get more information on the different sized aggregate species in her sample.
Monday – 1pm
Polly: Hi Professor. I’ve got the NTA data you suggested. You were right – it was pretty quick and …