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Zetasizer

The world’s most widely used system for nanoparticle, colloid and protein size, zeta potential and molecular weight measurements.

Customer experience, Dynamic Light Scattering, Meet the Experts, Particle size, Zetasizer »

[20 Jun 2017 | ]

Three methods to verify dynamic light scattering systems
If you are a meticulous researcher you may want to test your new Zetasizer. Is it really providing the correct size? One of the advantages of dynamic light scattering is that there are no tweakable adjustments, it is all physics: laser wave length, scattering angle, refractive index of the dispersant are all known in advance and are given parameters for the optical setup and sample. Nevertheless, it is a good idea to check and verify the performance of the system. One of the …

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Customer experience, Dynamic Light Scattering, Electrophoretic light scattering, Molecular size, Particle size, Zeta potential, Zetasizer »

[25 May 2017 | ]

A challenge to all new Zetasizers!
 
The Zetasizer is so simple to use that the majority of Zetasizer (user)s just “dive in” and find out how to operate the system.
By design it is (almost) indestructable, so what can go wrong? Not much – nevertheless, you might be able to get even more use and benefit from your system with a little preparation.
 
Free eLearning video – really!
If you are relatively new to the Zetasizer then take a look at our free eLearning introductory course. It was meant as a teaser for the …

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Dynamic Light Scattering, Meet the Experts, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Zetasizer »

[18 May 2017 | ]

Matt McGann and I will be representing Malvern at the upcoming annual UD Biophysical Characterization Workshop, sponsored by CBST (Center for Biomanufacturing Science & Technology), BITC (Biomolecular Interaction Technologies Center), and the University of Delaware, on June 6, 2017, hosted by Professor Christopher Roberts.
This year’s workshop will consist of three days of short courses and small-group demonstrations of the principles and commercial technologies for characterization of protein solutions and related colloidal systems, focusing specifically on static and dynamic light scattering, electrophoresis (DLS, ELS), and Taylor Dispersion Analysis (TDA).
Matt has been …

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Archimedes - Particle Metrology System, Chemical identification, Fluorescence Detection, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Molecular size, Molecular structure, Molecular weight, Morphologi G3-ID, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Protein higher order structure, Protein Mobility, Viscosizer TD, Zeta potential, Zetasizer »

[16 May 2017 | ]

With Pre-Conference Workshop on Biophysical Characterization and Particle Analysis
 

Date: July 17th – 20th.
We at Malvern know that protein stability is important in numerous disciplines, ranging from basic and medical biochemistry to pharmaceutical sciences. However, it is rare that researchers from all the relevant areas can join to discuss the critical issues in the field. The 2017 Colorado Protein Stability Conference provides a unique forum for this exchange of information. The topics to be covered include protein folding, dynamics and thermodynamic stability; amyloidosis and protein aggregation; and stability, formulation, analysis and processing …

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Customer experience, Dynamic Light Scattering, Molecular size, Particle size, Zeta potential, Zetasizer »

[4 May 2017 | ]

3 stumbling blocks for Report Designer (Zetasizer) – the rule of two
The three most common holdups encountered in the report designer feature are listed here.
Not everyone delves into the depth of the Zetasizer software, but of those who do want to take advantage of the report customization features in the ‘report designer’ nearly everyone runs into at least one of these three common mistakes:

1. Folder locations – there are two!
The Zetasizer software is typically installed in a (hidden) Windows directory like  C:\Program Files\Malvern Instruments\Zetasizer Software and that’s good. However, if …

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Corporate, Customer experience, Dynamic Light Scattering, Inside Malvern, Zetasizer »

[25 Apr 2017 | ]

Exposition at scientific meetings:
How useful are they and can they help me to get a job?
A brief insight from the ACS meeting
If you follow my writings, you’d probably expect more detail about Dynamic Light Scattering (DLS)… However, today I’d like to report on a recent conference we went to, the national meeting of the American Chemical Society, otherwise known as ACS. It is the world’s largest scientific society by number with close to 160k members.  There were over 17k registrants at the San Francisco meeting earlier this month, quite a turnout. With the constant …

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Dynamic Light Scattering, Electrophoretic light scattering, Meet the Experts, Static Light Scattering, Zeta potential, Zetasizer »

[16 Mar 2017 | ]

Recently, I hosted a webinar which discussed what surface zeta potential is and how it can be measured. The webinar attracted huge interest and it was a pleasure to see so many people attending and asking some excellent questions. In fact, there were so many questions asked, that we ran out of time at the end of the webinar and had to answer many of them via e-mail. Some of these are listed below.
The webinar discussed the zeta potential of solid, planar surfaces (rather than colloidal dispersions or molecular solutions) …

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Français, Meet the Experts, Zeta potential, Zetasizer »

[13 Mar 2017 | ]

Les deuxièmes ateliers de l’IFTS (Institut de Filtration et des Techniques Séparatives) auront lieu les 15 et 16 mars à Foulayronnes (près d’Agen).
Cette année, ces ateliers seront dédiés à la caractérisation des boues et suspensions.
Le mercredi 15 mars, de 15H15 à 15H30, Michel Terray et Alain Blasco présenteront le Zetasizer WT, appareil pour la mesure en ligne du potentiel zêta lors d’une conférence intitulée “Process control of water treatment and filtration by zeta potential measurement” contenant :

Intérêt du potentiel zêta dans le suivi de floculation
Exemple d’application chez Wemlinger et Severn Trent Water
Retour d’expérience basé sur …

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Dynamic Light Scattering, Molecular size, Particle concentration, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[7 Mar 2017 | ]

How can I use DLS for sample xyz?
What sample concentration / refractive index is needed for reliable DLS size measurements?

Has it been done before?
In Dynamic Light Scattering (DLS) intensity fluctuations in the sample are analyzed to obtain size information on the sample. The technique is applicable to a wide size range from sub-nanometer up to a few microns. In order to obtain a meaningful result, a minimum scattering signal strength must be present. Malvern’s Zetasizer Nano software contains a calculator to help estimate the required minimum concentration.
If anyone else has …

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Meet the Experts, Molecular weight, OMNISEC, Particle size, Tech Talk, Zeta potential, Zetasizer »

[30 Jan 2017 | ]

Malvern Instruments takes part at the 8th International Symposium on the Separation and Characterization of Natural and Synthetic Macromolecules (SCM-8) at Rhone Events & Congrescenter. The main interest of the conference is the separation and characterization of large molecules. The target audience includes specialists from analytical chemistry, polymer science, and life-science communities.
Malvern is a sponsor of the conference so keep an eye out for us at our booth. In addition, I will give a talk on Thursday February, 2nd about ‘Combining Multi-Detector GPC Measurements with Ultra-High Performance GPC Separations’. The …

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Dynamic Light Scattering, Particle size, Zetasizer »

[23 Jan 2017 | ]

Over the years one of the frequently asked questions that stands out in the data analysis of dynamic light scattering results is the issue about which size one should report.
 
 
 
Here is an example of just such an email I received this month:
“We have several data sets with low PDI (<0.1), single exponential decay, low cumulant fit error(~10^(-4)).  The intensity-distribution size and the cumulant fit size are similar.  But the number-distribution size (d=33 nm)and size obtained by cumulant fit(d=47 nm) are very different.  In this case, should we trust the number-distribution size …

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Electrophoretic light scattering, Protein Mobility, Tech Talk, Zetasizer »

[12 Jan 2017 | ]

Electrophoretic mobility measurements from proteins: How to protect proteins in an electric field
 
What is the protein mobility mode?
When applying an electric field to a sample containing protein molecules, there are a few considerations to take into account to avoid “over-stressing” delicate biomolecules. The force a charged object experiences in an electric field is proportional to its charge times the electric field. Therefore the electric field strength (defined by the applied voltage divided by the distance between the electrodes, in V/cm) is more relevant than the absolute value of the applied …

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Dynamic Light Scattering, Molecular size, Molecular structure, Particle size, Tech Talk, Zetasizer »

[27 Dec 2016 | ]

How to measure it with DLS?
What virus concentration is needed for reliable DLS size measurements?
 
What is a virus?
In cell biology, a virus is a self assembly of organic molecules (mainly proteins) in the size range 20 nm – 300 nm (diameter). Viruses hold great interest for human gene therapy researchers, and the AAV-2  virus shown to the left is an example.
How to size a virus in solution?

One of the techniques available to size viruses (as well as other biological nanoparticles) is Dynamic Light Scattering (DLS), where intensity fluctuations in a small …

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Inside Malvern, Zetasizer »

[9 Dec 2016 | ]

It’s lovely to work in the Malvern Instruments Headquarter at this time of year.  Our usually functional but slightly dull open plan offices are festooned with festive decorations, as each team and department tries to out-do the rest with the flashiest and gaudiest display possible. Of all the departments at Malvern, NPI (New Product Introduction) has really gone to town this year.  Maybe that’s because they have a secret weapon…. snazzy 3D printed, all singing, all dancing, exclusively designed decorations.
For the second year running, Rowena Innocent, Engineering Director, had organised a friendly competition to …

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Dynamic Light Scattering, Français, Laser Diffraction, Malvern Events, Meet the Experts, Nanosight, Particle size, Static Light Scattering, Zetasizer »

[23 Nov 2016 | ]

Les 20èmes rencontres organisées à Rennes auront lieu du 28 au 30 novembre avec la collaboration l’Institut des Sciences Chimiques de Rennes et la plateforme ScanMat. La directrice de cette plateforme, Maryline Guilloux-Viry, nous fera l’honneur d’accueillir les participants et de présenter les moyens et compétences disponibles au sein de la fédération des 5 plateformes constituant ScanMat (Microscopies électroniques, Analyses structurales et élémentaire, Synthèse et mise en forme, Spectroscopies et Microspectroscopies optiques).
Les nanoparticules seront au cœur de ces rencontres avec une évocation des nouvelles réglementations de REACH qui seront en vigueur …

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Malvern Events, Zetasizer »

[8 Nov 2016 | ]

When it comes to maintaining a stable water treatment operation, the right coagulant dose is critical for efficient flocculation. Traditional methods for setting this parameter are imprecise and labor intensive, and as a result, over- and under-dosing is common and response to process changes is reactive at best.
At the American Water Works Association’s (AWWA) Water Quality Technical Conference (WQTC) in Indianapolis, on November 13-15, Malvern will be showcasing its Zetasizer WT which helps water treatment plants better manage coagulation control.
The Malvern Zetasizer WT provides unique benefits unavailable by other methods:

Fully automated …

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Dynamic Light Scattering, Molecular size, Protein Aggregation, Tech Talk, Zetasizer »

[1 Nov 2016 | ]

α-synuclein promotes vesicle assembly – when size can support theory
 

What is a vesicle?
In cell biology, a vesicle is an (often spherical) assembly of amphiphilic molecules (usually proteins and phospholipids), and is typically sized between 30 nm – 100 nm in diameter. Vesicles are often classified by the type of interaction they are involved with. For extracellular vesicles (or exosomes), this is usually intercellular signal transmission. A particular example of exosomes is synaptic vesicles, which transmit signals between neurons (average size: 40 nm).
In a paper entitled “Structural basis of synaptic vesicle assembly promoted …

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Customer experience, Dynamic Light Scattering, Electrophoretic light scattering, Molecular size, Molecular weight, Particle size, Zeta potential, Zetasizer »

[20 Oct 2016 | ]

How can I save paper and print a Zetasizer report as a PDF?
 
Why print?
The Zetasizer software includes several popular report options. The most frequently used of these is probably the Intensity PSD (M), which gives a compact overview of the results. It lists the z-average and up to three peaks by intensity with their respective mean size, the area under the peak in % intensity and the standard deviation of the peak. In addition, several relevant sample identifiers and measurement conditions, like the sample name,  file name, refractive index of …

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Customer experience, Meet the Experts, Zeta potential, Zetasizer »

[13 Oct 2016 | ]

Water Treatment
The 7th International Conference and exhibition on Water Wastewater and Environmental Monitoring (WWEM) will take place at Telford, England on 2-3 November 2016.
We are giving a workshop on Thursday 3rd at 11:00 (in room 7).
This is “A brief introduction to zeta potential and sizing techniques in the water industry” and will mainly be focused on zeta potential which is gaining popularity as a monitor of particle instability to optimize the addition of expensive treatment chemicals, ultimately saving money.
Seasonal and geographical variation means the amount of chemical coagulant that needs …

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Dynamic Light Scattering, Electrophoretic light scattering, Meet the Experts, Particle size, Protein Aggregation, Zeta potential, Zetasizer »

[11 Oct 2016 | ]

Adjuvants for vaccination – How size and zeta may be relevant
 
What is an adjuvant?
Adjuvants are substances which are added to medicines to trigger and enhance an immune response to an antigen. In the USA, the most commonly-used adjuvant is alum, and many vaccines in use today contain some aluminum components to help the vaccine work better. While the exact mechanisms of how adjuvants work is unclear to some extent, a typical adjuvant component is much larger than the antigen or antibody itself, often around 100 nm in radius.
In a paper entitled …

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