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Differential Scanning Calorimetry

Differential Scanning Calorimetry (DSC) is a technique used to characterize the stability of a protein or other biomolecule directly in its native form. It does this by measuring the heat change associated with the molecule’s thermal denaturation when heated at a constant rate.

Differential Scanning Calorimetry, Dynamic Light Scattering, Meet the Experts, MicroCal, Molecular size, Molecular weight, OMNISEC, Particle concentration, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Zetasizer »

[18 Jul 2017 | ]

Monoclonal Antibodies – or mAbs for short

Since many of these mAbs require refrigeration we had used dynamic light scattering (with a Zetasizer) to show the formation of aggregates after freeze-thaw cycles (for details see application note “Effect of storage conditions on Immunoglobulin“). These could be seen both in the increased polydispersity index (PDI) and the appearance of a large-size aggregate peak in the intensity size distribution as shown to the right. The area under the peak gives an indication of the presence of the large aggregates but is not suited for …

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Differential Scanning Calorimetry, Meet the Experts, MicroCal, Microcalorimetry, Protein higher order structure »

[4 Jul 2017 | ]

Protein Antigens and DSC

This blog highlights two recent articles that focus on the use of a MicroCal Differential Scanning Calorimetry (DSC) system.
Differential Scanning Calorimetry – A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
I.B. Duruwoju, K.M. Bhandal, J. Hu, B. Carpick, and M. Kirkitadze
Journal of Visualized Experiments (JoVE), Issue 121, e55262, doi:10.3791/55262 (2017)
The authors (from Sanofi Pasteur in Toronto, Canada), provide a detailed protocol to perform Differential Scanning Calorimetry experiments on an automated DSC, such as a MicroCal VP-Capillary DSC system from Malvern Instruments.  This piece is a JoVE …

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Archimedes - Particle Metrology System, Customer experience, Differential Scanning Calorimetry, Gel permeation chromatography, Image Analysis, MicroCal, Morphologi G3-ID, Nanoparticle Tracking Analysis, Nanosight, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Viscotek »

[1 Jun 2017 | ]

Our key driver at Malvern Instruments is to create technologies that make a difference or solve a problem, and working hand-in-hand with our customers enables us to make sure that’s exactly what’s happening, out in the field.  We place a high value on our collaborations and partnerships with customers from a wide range of industries and organizations across the globe and love to see how our systems are being used in real-world scenarios.  This gives us vital feedback on what we’re doing well, and also any tweaks we need to make in order to optimize …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, MicroCal, Microcalorimetry, Molecular weight, Tech Talk »

[23 May 2017 | ]

A professional and personal view
If your newsfeed from scientific and mainstream media sources is anything like mine, you have seen numerous articles and blogs about the ‘reproducibility crisis’ in the life sciences.
Well-established cornerstones of scientific inquiry and research are the publication of findings in peer-reviewed journals, along with the opportunity for others to reproduce and verify published data.  This advancement and consolidation of knowledge leads to further understanding on how biological systems work, and provides the basis for the discovery and development of new drugs which benefit all of us.
However, there …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[11 May 2017 | ]

In September 2016 Malvern Instruments co-organized a European MicroCal meeting with Institut Pasteur and ARBRE European molecular biophysics network. The meeting was very successful and sparked animated discussions during scientific presentations, panel and poster sessions and interactive workshops.  A key focus was on best practice for experimental design and data analysis to ensure high-quality data and informed decisions that help drive scientific projects forward.
In two days, together with more than 120 participants from 19 countries, we covered a wide range of topics, from interaction and kinetic analysis with ITC to …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Protein higher order structure »

[1 Mar 2017 | ]

The NEW MicroCal PEAQ-DSC – the gold standard for thermal stability determination
Differential scanning calorimetry (DSC) is an established biophysical tool for protein stability characterization during drug development. DSC data are used to advance the most stable and ‘developable’ proteins into the pipeline, and optimize the process and formulation conditions which will maintain protein stability during manufacturing and storage. DSC data are also important in higher order structure (HOS) protein characterization, lot-to-lot comparability, and characterization of biosimilar drugs compared to innovators.
Our previous technology, the MicroCal VP-Capillary DSC Automated system, is a …

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Binding affinity and Kd, Differential Scanning Calorimetry, Dynamic Light Scattering, Français, Gel permeation chromatography, Image Analysis, Isothermal Titration Calorimetry, Laser Diffraction, Meet the Experts, Molecular size, Nanoparticle Tracking Analysis, Particle concentration, Particle size, Rheological and Viscosity, Rheometry - Rotational, Size Exclusion Chromatography, Static Light Scattering, Zeta potential »

[21 Feb 2017 | ]

Afin de vous apporter un support concret pour utiliser votre système de façon optimale, finaliser vos bonnes pratiques de laboratoire et répondre à vos besoins spécifiques, Malvern organise des formations sur site ou en sessions dans nos 3 agences françaises (Orsay, Lyon et Toulouse).
Voici les dates de toutes les sessions programmées pour 2017 :
CARACTERISATION DES PARTICULES PAR DIFFRACTION LASER
Objectifs : Perfectionner les méthodes de caractérisation des particules en voie sèche et liquide, définir les bonnes pratiques de laboratoire et s’adapter aux besoins spécifiques de chaque installation. Comprendre le principe physique …

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Differential Scanning Calorimetry, Malvern Events, MicroCal, Tech Talk »

[14 Feb 2017 | ]

My introduction to Differential Scanning Calorimetry (DSC) was a postdoctoral fellow in the late 1980s.  I was producing mutant forms of the enzyme thymidylate synthase, and I needed to characterize the effects amino acid changes had on the overall stability of the protein.  Using a DSC instrument at our facility, I compared the thermal stability of the different mutants to wild-type protein by ranking the thermal transition temperature (TM). As I recall, I needed about 2 mg -4 mg protein per DSC experiment, which was a lot of protein at …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Events, Meet the Experts, MicroCal, Microcalorimetry, Protein higher order structure »

[2 Feb 2017 | ]

We are proud to announce Malvern Instruments’s Gold Sponsorship of Global Engage’s Biosimilars and Biologics Congress 2017.  This two-day event will be taking place on Monday 6th and Tuesday 7th March 2017, at the Leonardo Royal Hotel Berlin Alexanderplatz.
This meeting will be attended by industry, regulatory and academic experts from all areas of protein, antibody and biosimilar research and will comprise three topic streams: Innovations in Antibody-based Therapeutics; Expression, Purification and Characterization (with a particular focus on product stability and half-life); Biosimilars.
Natalia Markova, Principal Scientist, Malvern MicroCal, will be discussing …

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Differential Scanning Calorimetry, Label-free analysis, Malvern Events, Meet the Experts, MicroCal, Microcalorimetry, Protein higher order structure »

[31 Jan 2017 | ]

DSC for the regulated environment
 

 
Advancing biotherapeutic molecules along the drug development pipeline is a complex, time-consuming and expensive process. Good stability is key to a candidate’s progression, and must be assessed and reviewed throughout development – from candidate selection, through formulation development, all the way to batch-to-batch comparability or biosimilarity assessment….and beyond! Objective, reliable stability data is needed for confident decision-making about which drug products and processes to move through development into commercialization.
Differential Scanning Calorimetry (DSC) is a biophysical tool for protein stability characterization. The MicroCal VP-Capillary DSC system, launched in 2002, …

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Customer experience, Differential Scanning Calorimetry, Dynamic Light Scattering, Français, Isothermal Titration Calorimetry, Meet the Experts, Nanoparticle Tracking Analysis, Tech Talk »

[14 Dec 2016 | ]

Journée d’information APGI/MALVERN – “Nouvelles méthodes de caractérisations des biomolécules”

Mercredi 11 janvier 2017 au CNAM
Co-organisé avec APGI et le CNAM
Avec le développement rapide de biomolécules dans les nouvelles thérapies, des techniques de caractérisation innovantes se développent en parallèle des techniques classiques (comme par exemple la chromatographie d’exclusion couplées à de la triple ou tetra détection, ou la technique NTA, très adaptée à la mesure des exosomes, des particules subvisibles et tout autre type de contaminant, etc).
C’est pourquoi l’APGI (Association de Pharmacie Galénique Industrielle) et Malvern vous invitent à une journée …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Events, MicroCal »

[9 Nov 2016 | ]

I always enjoy meeting our Malvern customers whenever I travel to different sites and events. I have been assisting customers with MicroCal ITC (Isothermal Titration Calorimetry) and DSC (Differential Scanning Calorimetry) instruments for over 15 years, and there are customers I have known since 2001. Every time I think I “know it all” I always learn something new from our customers, such as a unique sample analyzed using our instruments, or a “trick” for sample preparation or data analysis.
Malvern Instruments recently hosted two MicroCal “User Days” in San Diego, CA …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Events, Meet the Experts, MicroCal »

[5 Oct 2016 | ]

Onto Day 2 of the meeting in the beautiful sunshine of Paris, with another agenda full to brimming with a great set of inspiring presentations.
The first session of the morning was chaired by Malvern’s Aymeric Audfray, and was entitled: “Developments in ITC applications into challenging areas”. The session was kicked off by Anne Imberty, who spoke about “ITC for structural glycosciences tools”.  This talk was followed by Laurent Bouteiller from Pierre and Marie Curie University who delivered a presentation on using “ITC as a tool for the characterization of self-assembled systems in organic solvents”.
Interaction with the …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Events, MicroCal »

[27 Sep 2016 | ]

Patrick England from the Institut Pasteur in Paris opened the Second European MicroCal meeting by welcoming all of the 100+ delegates, on behalf of the hosts and organisers: Institut Pasteur,  the Arbre committee and Malvern Instruments.
 
 
”I liked the workshop session and that the meeting was very interactive. The venue was great and easily accessible.” Blanca Lopez, Copenhagen University
Morning session
The day started with Geoff Holdgate kicking off the interaction analysis session, his talk centering on the value of using an orthogonal approach, and describing his use of a MicroCal Isothermal Titration Calorimeter for …

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Binding affinity and Kd, Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Protein higher order structure »

[23 Aug 2016 | ]

Malvern Instruments is sponsoring two “MicroCal User Days” in October:

Tuesday October 18 2016: West Coast MicroCal User Day in San Diego, CA
This complimentary 1-day event will include presentations by scientists discussing how ITC and/or DSC are used as part of their research.

Invited speakers:
Bill Callahan III, Ph.D., Senior Scientist, Amgen Thousand Oaks, Characterizing Amorphous Suspensions: Potential Formulation Option for Monoclonal Antibodies
Matthew McGann, Product Manager, Innovative Products, Malvern Instruments, Assessing Bioformulation Stability – From prediction to characterizing particle content

Thursday October 27 2016: New England MicroCal User Day in Cambridge, MA
Come hear from …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Events, Meet the Experts, MicroCal, Microcalorimetry »

[29 Jul 2016 | ]

In keeping with our long tradition of working hand-in-hand with our customers, Malvern MicroCal is arranging the second of our annual European meetings to bring together current calorimetry users with all those interested in ITC and DSC technologies and applications – this will take place on September 26th-27th in Paris.
This year we are pleased to be co-organizing the meeting with Institut Pasteur (who will be hosting the meeting) and ARBRE European molecular biophysics network.
 
The agenda of the meeting encompasses a wide range of calorimetry application areas, from interaction and kinetic analysis …

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Differential Scanning Calorimetry, Malvern Events »

[26 Jul 2016 | ]

Digging Deeper with Differential Scanning Calorimetry to Study Key Protein Characteristics 

Please join us for a webinar presented by Dr Katherine Bowers (Fujifilm Diosynth Biotechnologies USA Inc.) for an informative webinar on how Differential Scanning Calorimetry (DSC) is used in protein characterization.

 
 
DSC has a long history of playing a vital role in protein biophysical studies as well as in biopharmaceutical development. From candidate selection, formulation development, stability monitoring and bio-similarity assessment, DSC still remains one of the top assays utilized in the pharmaceutical industry during the development and approval of protein-based …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Events, MicroCal, Microcalorimetry »

[31 May 2016 | ]

Microcalorimetry is hot in Brazil!
In February 2016, I participated in microcalorimetry workshops in Rio de Janeiro and Sao Carlos, Brazil. There is a long-standing and active group of calorimetry users in Brazil, and I had the opportunity to give introductory isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) presentations at each workshop. Faculty members and students presented their research projects including ITC and/or DSC results.
 
 
These workshops were coordinated by my Malvern Brazil colleague, Henrique Kajiyama. During our Brazilian tour, Henrique and I had the opportunity to speak to attendees …

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Differential Scanning Calorimetry, Electrophoretic light scattering, Isothermal Titration Calorimetry, Label-free analysis, Malvern Events, MicroCal, Microcalorimetry, Molecular structure, Protein Aggregation, Raman spectroscopy, Zetasizer »

[12 May 2016 | ]

I had the pleasure of participating in the 5th International Symposium on Higher Order Structure of Protein Therapeutics, also known as HOS2016, sponsored by CASSS. The meeting was at the Renaissance Hotel in Long Beach, California April 11-13, 2016. I was joined by my Malvern colleagues Rajib Ahmed and David Sanborn.
The HOS Symposium is a platform for open discussion of development and optimization of analytical techniques to study HOS of proteins and biopharmaceutical products (including monoclonal antibodies and antibody-drug conjugates), biocomparability, and biosimilars.
Protein higher order structure (HOS) includes the secondary, …

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Differential Scanning Calorimetry, Meet the Experts, MicroCal »

[15 Mar 2016 | ]

During the development of proteins for use as biopharmaceuticals, the primary structure (amino acid sequence) is important in defining protein activity. Due to the complex nature of protein drugs, compared to small molecule drugs, biophysical techniques are important tools in the characterization of the protein’s higher-order structure (HOS) to understand the protein’s stability, folding, structure, and functional activity. HOS is characterized by a variety of biophysical methods including:

Mass spectrometry (MS)
Circular dichroism (CD)
Fourier transform infrared spectroscopy (FT-IR)
Raman spectroscopy
X-ray crystallography
Nuclear magnetic resonance (NMR)
Near-UV CD
Size exclusion HPLC
Fluorescence
Static and dynamic light scattering (SLS and …

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