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Dynamic Light Scattering

Dynamic light scattering (DLS), sometimes referred to as Quasi-Elastic Light Scattering (QELS), is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1nm. Dynamic light scattering (DLS) is often used to determine the size of particles in suspension and or polymers in a solution. We cover off a number of dynamic light scattering (DLS) articles here in our ever growing dynamic light scattering (DLS) blog category.

Customer experience, Dynamic Light Scattering, Malvern Panalytical Events, Meet the Experts, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Zeta potential, Zetasizer »

[30 Aug 2018 | ]

Malvern Panalytical Webinars

Here is the list of the upcoming webinars in the coming weeks. You can register for free to all our webinars and you will automatically receive the On Demand version if you can’t finally attend the live event directly in your inbox!
September
 
6th – Quality by Design: Principles for pharmaceutical method development
Steve Ward-Smith will outline what QbD is, how it works within pharmaceutical development, and will provide some examples of the ‘fishbone’ diagrams which show the factors that should be considered when developing methods for laser diffraction and dynamic light scattering.
11th – Adaptive Correlation: …

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Dynamic Light Scattering, Electrophoretic light scattering, Malvern Panalytical Events, Particle size, Zetasizer »

[9 Aug 2018 | ]

Launching the latest Zetasizer in Mexico!
 
June was a special month at our Mexico City office. We held a specific launch party organized for the latest addition to our Zetasizer family of instruments – the Zetasizer Pro and the Zetasizer Ultra. One of the groundbreaking novelties in the new system is adaptive correlation, a new method to perform dynamic light scattering (DLS) measurements, where rapid correlation functions are classified as being steady state or transient. Steady-state data sets describe particles that are consistently part of the measurement volume and hence characteristic of …

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Dynamic Light Scattering, Malvern Panalytical Events, Molecular size, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Zetasizer »

[7 Aug 2018 | ]

Workshop of the LOEWE Center for Translational Medicine and Pharmacology
20th September 2018
Campus Riedberg BIOLOGICUM, Lecture hall 1, -1.202
 
As market interest and investment in nanotechnology surges and the requirements for novel formulation strategies continues to grow, the industry is under pressure to provide a detailed characterization of subvisible particle species and their interactions with biological systems.
Formulation development and manufacture of biopharmaceuticals and nanomaterials are lengthy and complex processes with very specific analytical challenges. We know that in order to successfully deliver such therapies to market it is vital to reduce risk as …

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Dynamic Light Scattering, Molecular size, Particle concentration, Particle size, Tech Talk, Zeta potential, Zetasizer »

[2 Aug 2018 | ]

 
Thanks to all who attended the launch webinar for Malvern Panalytical’s new Zetasizer systems, and special thanks to Mike Kaszuba for presenting a clear and interesting overview of the two instruments – we hope you all enjoyed it and found it interesting.

The launch of the Zetasizer Pro and Zetasizer Ultra received a great response and naturally, due to the innovations included in the new systems, lots of questions were sent to us both during and after the webinar. We’ve taken the opportunity to answer the most frequently-asked questions here, in …

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Corporate, Dynamic Light Scattering, Zeta potential, Zetasizer »

[17 Jul 2018 | ]

Severn Trent and Malvern Panalytical share award for the development of an online Zetasizer for fully automated coagulation control
Great news for the fans of zeta potential in water treatment! Zetasizer WT, the first commercial on-line zeta potential analyzer has been awarded a first place by the UK water industry in the category of “Most innovative use of existing technology”. The project titled “Online Zetasizer for fully automated coagulation control” is a collaboration between Severn Trent Water and Malvern Panalytical to enable a fully automatic control of coagulant dosing in a …

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Dynamic Light Scattering, Tech Talk, Zeta potential, Zetasizer »

[10 Jul 2018 | ]

Dynamic light scattering (DLS) is widely used for ensemble characterization of hydrodynamic particle size, but I have a number of frustrations with the technique.
We measure our valuable samples in low concentrations and in very small volumes, so we use expensive, low volume, glass cells, which are optically superior to disposable plastic cuvettes, but we spend far too much time cleaning them, significantly reducing the productivity of our group.
Also, our samples range from monodisperse proteins to polymeric nanoparticles to liposomes, ranging from the nano-scale into the micro-scale and we need to …

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Dynamic Light Scattering, Particle size, Tech Talk, Zeta potential, Zetasizer »

[28 Jun 2018 | ]

What causes the most headaches for a particle characterization scientist?
There are many answers to this question, but one of the most obvious is dust or contaminants! As someone who regularly uses Dynamic Light Scattering (DLS) to measure particle size, the possibility of there being dust or contaminants in my sample is one of the things I always bear in mind.
The way I prepare my sample, the way I store my sample before measurement, remembering to put the measurement cap on the cuvette, but only after I’ve cleaned it using an …

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Dynamic Light Scattering, Meet the Experts, Zeta potential, Zetasizer »

[14 Jun 2018 | ]

Have you ever tried to explain to someone that the large peak they can see in their dynamic light scattering data, that seems to dominate the size distribution by intensity – is actually only a very tiny part of the sample…and hardly ‘there’ at all?
This has happened to me on numerous occasions, and I always try to show the volume and number size distributions, illustrating how that peak disappears. However, there is often a lingering question hanging in the air: “But if we see it, then it’s there, right?”
In the …

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Binding affinity and Kd, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Isothermal Titration Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry, Molecular size, OMNISEC, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Viscosizer TD, Viscotek, Zetasizer »

[13 Jun 2018 | ]

The time is fast arriving for the third in our highly successful series of European MicroCal meetings! This year the meeting is organized by Malvern Panalytical alongside a Scientific Committee of expert users from academia and industry, all with the support of the ARBRE-MOBIEU network. Our venue will be the prestigious Institute of Engineering and Technology (IET), within the historical Savoy Place, perched on the north bank of the River Thames, with spectacular views across London.
The event will focus on applications, best practice, advanced data analysis and all the latest developments in both isothermal …

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Dynamic Light Scattering, Electrophoretic light scattering, Particle size, Tech Talk, Zeta potential, Zetasizer »

[31 May 2018 | ]

How good can a gold standard be?
 
When performing dynamic light scattering (DLS) measurements, we sometimes encounter especially nice data. An interesting issue to consider is the level of data quality one can expect to achieve, under ideal circumstances, when all other interferences are minimized. This is especially relevant at the smaller nanoscale where fewer standards are available, and to those who have less practical experience with sizing colloidal nanoparticles by DLS. How monodisperse can a good sample be? What are achievable polydispersity index (PDI) values to aspire to?
What is the …

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Corporate, Dynamic Light Scattering, Electrophoretic light scattering, Molecular size, Molecular weight, Particle concentration, Particle size, Zeta potential, Zetasizer »

[23 May 2018 | ]

Where is the Zetasizer used?
 
For around two decades, the Zetasizer range has been the de facto standard for performing dynamic light scattering (DLS) measurements on a wide range of particles and materials.
Today’s launch of the Zetasizer Pro and Zetasizer Ultra – which took place earlier today on May 23rd, 2018 – provides a good reason to summarize the key application areas for light scattering technology: both dynamic and electrophoretic! Where are these Zetasizers used? Where can we find them, in the real world?
Below is a list of key application focus areas:

Bioscience and biopharmaceuticals: …

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Customer experience, Dynamic Light Scattering, Molecular size, Particle size, Tech Talk, Zetasizer »

[15 May 2018 | ]

The peaks in your distribution are out of order?
We recently received an email about the order of peaks in the Zetasizer software:
For data evaluation, is it possible to rearrange the NNLS results by order of particle size populations?”
How the peaks are shown (by area)

Size measurement results are typically viewed in the Intensity PSD (M) tab. This is part of the standard Size and Summary workspaces.  When a record contains more than one peak, these results will be tabulated in the table above the intensity size distribution. The default ordering in …

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Dynamic Light Scattering, Malvern Panalytical Events, Meet the Experts, Zeta potential, Zetasizer »

[15 Mar 2018 | ]

Dynamic Light Scattering (DLS), sometimes referred to as Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering, is a technique classically used for measuring the size of particles typically in the sub-micron region, dispersed in a liquid. The sensitivity of some modern systems is such that it can also now be used to measure the size of macromolecules in solution.
In this new webinar, I summarise how the technique works, what its size and concentration limits are and discuss its advantages and disadvantages.
This webinar was well-attended and I received several questions, I thought …

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Dynamic Light Scattering, Gel permeation chromatography, Kinexus, Laser Diffraction, Malvern Panalytical Events, Meet the Experts, Molecular weight, Nanoparticle Tracking Analysis, Nanosight, OMNISEC, Particle concentration, Particle size, Rheology, Rheometry, Tech Talk, Viscotek, Zeta potential, Zetasizer »

[30 Jan 2018 | ]

We will be attending Pittcon 2018 in Orlando, Florida Feb 26th – Mar 1st and once again we are pleased to feature a number of our recognized characterization experts who will be presenting a variety of short courses throughout the week.
Time is running out, so don’t miss out on the early bird deadline for discounted admission which ends on February 5th.
Below is a listing of our available short course offerings.
We look forward to seeing you in Orlando at one of our short courses and our Booths #2118 & #2119.
Monday, February 26th
Fundamentals of Particle …

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Dynamic Light Scattering, Electrophoretic light scattering, Inside Malvern Panalytical, Zeta potential, Zetasizer »

[21 Dec 2017 | ]

On the 20th December 1977 (40 years ago) the Royal Radar and Signals Establishment (RRSE) and Malvern Instruments received the coveted MacRobert Award for the development of the Malvern Correlator.  This was fundamental to the development of Photon Correlation Spectroscopy (PCS) and the evolution of the Zetasizer product line. The MacRobert award is the UK’s longest-running and most prestigious national prize for engineering innovation. Other past winners include engineers behind innovations such as the Pegasus jet engine, catalytic converters, the roof of the Millennium Dome and intelligent prosthetic limbs.
The concept …

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Dynamic Light Scattering, Kinexus, Laser Diffraction, Mastersizer, Nanoparticle Tracking Analysis, Nanosight, Rheometry, Size Exclusion Chromatography, Tech Talk, X-Ray Diffraction (XRD), Zetasizer »

[5 Dec 2017 | ]

It was my great pleasure to present as part of the Worcestershire Innovation Network at their WINN Wednesday event on the 22nd of November, showcasing the best in innovation across Worcestershire.
This included a local company which makes artificial kneecaps and hips, an insight into the building of genomics studies of patients with rare diseases and cancers, an update on the appeal to get a state-of-the-art surgical robot for removal of prostate tumors (reducing discomfort, scarring and hospital stay post-operation). Jess Antley, head of WINN, also gave an update on a new shared space/collaboration …

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Differential Scanning Calorimetry, Dynamic Light Scattering, MicroCal, Molecular size, Molecular structure, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[16 Nov 2017 | ]

Measuring aggregation temperature using the Zetasizer

In dynamic light scattering (DLS), the hydrodynamic size and size distribution of particles in solution can be obtained.  It may be of interest to examine this measurement as a function of time and temperature, or as a ‘trend’, as it is called in the Malvern Zetasizer software.  For biological protein samples, this temperature trend is particularly interesting: although at low temperatures a protein may be stable and show repeatable size (and scattering intensity) measurements, typically at some elevated temperature (Tagg), protein molecules will show a tendency …

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Dynamic Light Scattering, Molecular size, Molecular weight, Static Light Scattering, Zetasizer »

[2 Nov 2017 | ]

Molecular weight measurements with the Zetasizer software?
First a few words of caution: molecular weight or Mw measurements in general are not easy, require time, good sample preparation skills as well as patience. If you are new to the Malvern Zetasizer it might be best to familiarize yourself first with the rapid, user-friendly, and quite forgiving technique of dynamic light scattering (DLS). {{If you are experienced and want to skip to a Brainshark narrated screen recording of the steps, best viewed in Chrome, go ahead to https://www.brainshark.com/malvern/sls-with-zetasizer }}.
OK, with that out …

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Dynamic Light Scattering, Particle size, Tech Talk, Zetasizer »

[26 Sep 2017 | ]

Is there a maximum viscosity for DLS measurements?
 
The Malvern Zetasizer can determine particle size by dynamic light scattering (DLS). In this technique, the intensity fluctuations are analyzed to find the corresponding diffusion coefficient that led to the fluctuations. The translational diffusion coefficient Dt of a particle is inversely related to its size and the viscosity of the dispersant (or more accurately the hydrodynamic radius rH and viscosity η).

This relationship can be used to predict detectability limits of the technique in general, and this is how we explore the idea of …

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Archimedes, Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Image Analysis, Label-free analysis, MicroCal, Microcalorimetry, Morphologi, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Rheology, Size Exclusion Chromatography, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Viscotek, Zetasizer »

[21 Sep 2017 | ]

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was a range of scientists and researchers from all over the world, with one common subject in mind: Protein Stability.

One of the highlights of this conference was the pre-conference workshop. This was a free workshop for all attendees of the conference, which was sponsored by Malvern Panalytical. The workshop included a range …

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