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Nanoparticle Tracking Analysis

Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension.

Binding affinity and Kd, Differential Scanning Calorimetry, Dynamic Light Scattering, Français, Gel permeation chromatography, Image Analysis, Isothermal Titration Calorimetry, Laser Diffraction, Meet the Experts, Molecular size, Nanoparticle Tracking Analysis, Particle concentration, Particle size, Rheological and Viscosity, Rheometry - Rotational, Size Exclusion Chromatography, Static Light Scattering, Zeta potential »

[21 Feb 2017 | ]

Afin de vous apporter un support concret pour utiliser votre système de façon optimale, finaliser vos bonnes pratiques de laboratoire et répondre à vos besoins spécifiques, Malvern organise des formations sur site ou en sessions dans nos 3 agences françaises (Orsay, Lyon et Toulouse).
Voici les dates de toutes les sessions programmées pour 2017 :
CARACTERISATION DES PARTICULES PAR DIFFRACTION LASER
Objectifs : Perfectionner les méthodes de caractérisation des particules en voie sèche et liquide, définir les bonnes pratiques de laboratoire et s’adapter aux besoins spécifiques de chaque installation. Comprendre le principe physique …

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Dynamic Light Scattering, Gel permeation chromatography, Malvern Events, Meet the Experts, Nanoparticle Tracking Analysis, Size Exclusion Chromatography, Zeta potential »

[26 Jan 2017 | ]

We will be attending Pittcon 2017 in Chicago, Illinois March 5th – 9th and once again we are pleased to feature a number of our recognized characterization experts who will be presenting a variety of short courses throughout the week.
Time is running out, so don’t miss out on the early bird deadline for discounted admission which ends on February 18th.
Below is a listing of our available short course offerings.
We look forward to seeing you in Chicago at one of our short courses and our Booth #2416.
Sunday, March 5, 2017 – 8:30 AM
Light Scattering …

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Customer experience, Differential Scanning Calorimetry, Dynamic Light Scattering, Français, Isothermal Titration Calorimetry, Meet the Experts, Nanoparticle Tracking Analysis, Tech Talk »

[14 Dec 2016 | ]

Journée d’information APGI/MALVERN – “Nouvelles méthodes de caractérisations des biomolécules”

Mercredi 11 janvier 2017 au CNAM
Co-organisé avec APGI et le CNAM
Avec le développement rapide de biomolécules dans les nouvelles thérapies, des techniques de caractérisation innovantes se développent en parallèle des techniques classiques (comme par exemple la chromatographie d’exclusion couplées à de la triple ou tetra détection, ou la technique NTA, très adaptée à la mesure des exosomes, des particules subvisibles et tout autre type de contaminant, etc).
C’est pourquoi l’APGI (Association de Pharmacie Galénique Industrielle) et Malvern vous invitent à une journée …

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Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[15 Sep 2016 | ]

Nanoparticle Tracking Analysis or Dynamic Light Scattering ?
After a recent joint NanoSight and Zetasizer demonstration, I received this set of questions that helps illustrate the core of the issue of these two techniques:

Since the Nanosight gives me particle concentration, it’s somewhat straightforward to calculate what % of my Nanoparticles are aggregated. Can I calculate % aggregated particles in the DLS as well?  Or does the varying amount of intensity, and the protein+nanoparticle mixture in the sample confound that calculation?

If I wanted to measure non-spherical particles (for example nanorods), which system …

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Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle size, Protein Aggregation, Tech Talk »

[26 May 2016 | ]

We recently published our application note, “Adeno-Associated Virus titer and aggregation characterization“, which discusses a new NanoSight method that allows the visualization and therefore analysis of viruses smaller than what was previously possible to measure.  This represents an exciting extension of the Nanoparticle Tracking Analysis (NTA) technique in response to market demand for tools for better characterization of small virus types.
 
Adeno-Associated Virus (AAV) is commonly used in the gene therapy field as a delivery vector. Tools to characterize both the concentration and purity or aggregation of the samples are limited because the primary virus size is …

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Isothermal Titration Calorimetry, Meet the Experts, Nanoparticle Tracking Analysis, Rheometry - Rotational, Size Exclusion Chromatography »

[5 Apr 2016 | ]

How time flies! Spring is here already! Just for you I have collated the latest list of our On Demand webinars. Just click and choose your favorite subject.
I also listed all our upcoming webinars for April. You can register for free and if you can’t attend, we’ll send you the recording few days after the live event so don’t hesitate, click now!
 
March On Demand webinars:

How Size Exclusion Chromatography can improve processes and quality in biopharmaceutical development
Applications of light scattering in pharmaceutical development
Interpretación de resultados de tamaño de partícula por la técnica de difracción …

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Malvern Events, Meet the Experts, Nanoparticle Tracking Analysis, Nanosight »

[21 Apr 2015 | ]

Here at Malvern, the NanoSight team is preparing for the International Society of Extracellular Vesicles (ISEV) meeting this week in Washington, D.C. and can’t wait to attend what will be the biggest extracellular vesicle conference to date. The widespread use of NanoSight systems in exosome and microvesicle research makes this one of our best chances to interact closely with users in this application space.  We will have three technical specialists on site to field any questions you have regarding the use of the Nanoparticle Tracking Analysis (NTA) technique for vesicle …

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Dynamic Light Scattering, Malvern Events, Meet the Experts, Nanoparticle Tracking Analysis, Nanosight, Particle size, Zeta potential, Zetasizer »

[6 Mar 2015 | ]

Malvern Instruments is heading to Pittcon 2015 this weekend in New Orleans, and while a lot of our time and energy is focused on making sure people get a great experience when visiting our booth (#3,545) or attending our short courses, we’re always keen to see what else is going on in the great conference and networking program. While preparing material for next week, I took a look at the conference program and was excited to see the description of the Wallace H. Coulter lecture that Naomi J. Halas, who …

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Dynamic Light Scattering, Meet the Experts, Nanoparticle Tracking Analysis, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Size Exclusion Chromatography, Tech Talk »

[24 Feb 2015 | ]

I recently took part in a question and answer session with BioPharm International about techniques for measuring and characterizing protein aggregates. The full discussion can be viewed here, however I thought I would also use this blog post to summarize the main points.
Essentially, there is a pressing need within the biopharmaceutical industry to understand aggregation pathways, and the risk factors that can induce aggregation, right from the start of the drug development process to align manufacturing processes with Quality by Design (QbD) principles. Given the link between protein aggregation and …

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Dynamic Light Scattering, Electrophoretic light scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Tech Talk, Zeta potential, Zetasizer »

[13 Jan 2015 | ]

The Consumer Electronics Show (CES) took over Las Vegas once again last week, and this time, quantum dots are the hottest thing happening in TV technology.  At CES 2015 LG revealed a 4K quantum dot TV, the UF9400, quickly followed by Samsung‘s own line of Ultra HD TVs featuring quantum dots.  Sony was one of the first tech companies off the mark, including quantum dot technology in some of its high end TVs back in 2013.
Quantum dots are uniquely highly scattering nanoparticles that can be used for image enhancement.  These light-emitting semiconductor nanocrystals …

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Archimedes - Particle Metrology System, Dynamic Light Scattering, Molecular weight, Nanoparticle Tracking Analysis, Nanosight, Particle size, Resonant mass measurement, Tech Talk, Zeta potential, Zetasizer »

[5 Nov 2014 | ]

Having spent more than twenty years in the instrumentation arena and worked with a variety of different characterization techniques in lots of different application spaces, I have sometimes struggled to explain to my family what it is that I do. Particularly to my two young children who have never quite got the intricacies of nanomaterial characterization despite my attempts to stress the importance by referring to everyday products like computers and iPods that they take for granted.
So it was within this context that I found myself generating a presentation on the important and evolving sector of …

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Archimedes - Particle Metrology System, Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Resonant mass measurement, Tech Talk, Zetasizer »

[2 Sep 2014 | ]

PhD student Polly came into the lab on Monday morning to find that her purified protein had started to aggregate after a weekend in the fridge. The profile she got from her Dynamic Light Scattering (DLS) measurement clearly showed two peaks, with the second in the micron range. Her supervisor suggested she tried Nanoparticle Tracking Analysis (NTA) to get more information on the different sized aggregate species in her sample. This showed that there were aggregates of many different sizes present. The question was, what should she do next?

Monday 3pm
Polly: …

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Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Tech Talk, Zetasizer »

[26 Aug 2014 | ]

PhD student Polly came into the lab on Monday morning to find that her purified protein had started to aggregate after a weekend in the fridge. The profile she got from her Dynamic Light Scattering (DLS) measurement clearly showed two peaks, with the second in the micron range. Her supervisor suggested she tried Nanoparticle Tracking Analysis (NTA) to get more information on the different sized aggregate species in her sample.
 
Monday – 1pm
Polly: Hi Professor. I’ve got the NTA data you suggested. You were right – it was pretty quick and …

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Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Tech Talk, Zetasizer »

[20 Aug 2014 | ]

Polly is a PhD student researching into cystic fibrosis. She had been working on a recombinant protein. On Friday, she finished the final purification step, ran a quick measurement by Dynamic Light Scattering (DLS) on the Zetasizer to check purity before storing the sample in the fridge for the weekend. Arriving back in work on Monday, Polly was eager to see how her protein was getting on….
Professor: How’s your newly purified protein after the weekend at 4°C? Have you re-run the DLS today – does it look the same as on Friday?
Polly: …

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Nanoparticle Tracking Analysis, Nanosight, Tech Talk »

[31 Jul 2014 | ]

We’ve just released NTA 3.0, a new version of the software that drives our NanoSight Nanoparticle Tracking Analysis (NTA) systems. I chatted to Jonathan Smith, Senior Software & Systems Leader here at Malvern, who is the brains behind the development of the new software.  He gave me the lowdown on what we can expect from the update.
What key things you were looking to achieve with NTA 3.0?
It was important to us to make the measurement procedure more consistent and clear for new or inexperienced users, so that our instruments are easier to use. This …

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Archimedes - Particle Metrology System, Nanoparticle Tracking Analysis, Nanosight, Resonant mass measurement, Tech Talk »

[22 Jul 2014 | ]

Last week I attended the 4th Microbubble Symposium hosted by the Leeds Microbubble Consortium at University of Leeds in the UK. The meeting focused on the Fabrication, Characterization and Translational Applications of Microbubbles. I went along to hear about the latest developments in this fascinating area of research and to share my ideas on how our Nanosight & Archimedes instruments could help.
Much of the UK work on Microbubbles was shown to be coming from The University of Leeds where they are developing (amongst other things) “theranostic” Microbubbles that double as …

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Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Tech Talk, Zetasizer »

[3 Jul 2014 | ]

Having just attended a nanotech conference in Washington in June, it was interesting to note that the FDA issued a number of guidelines within the same period for companies using nanotechnology in products regulated by the government. Broadly they issued three guidelines for industry which are based around:

whether an FDA regulated product involves the application of Nanotechnology

safety of Nanomaterials in cosmetic products

assessing the effects of significant manufacturing process changes, including emerging technologies

The general position within these documents is to ask some key questions that will indicate whether a product will …

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Malvern Events, Nanoparticle Tracking Analysis, Nanosight »

[24 Apr 2014 | ]

Here at Malvern, we are committed to sharing our experience and ideas with the people working on the front line of analytical science. So I am especially pleased to be travelling to Rotterdam for ISEV 2014, where I will present a paper – ‘The practical use of nanoparticle tracking analysis (NTA) to characterize extracellular vesicles in general and when using fluorescence markers.’
ISEV 2014 – a meeting of the International Society of Extracellular Vesicles – is taking place between the 30th April and the 3rd May. I’ll be presenting the paper, …

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