Resonant mass measurement
Resonant mass measurement is a technique used in an innovative way by Archimedes to detect and count sub visible and submicron particles in a sample, and to measure their size and mass and distributions of size and mass.
Dynamic Light Scattering, Meet the Experts, Nanoparticle Tracking Analysis, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Size Exclusion Chromatography, Tech Talk »
I recently took part in a question and answer session with BioPharm International about techniques for measuring and characterizing protein aggregates. The full discussion can be viewed here, however I thought I would also use this blog post to summarize the main points.
Essentially, there is a pressing need within the biopharmaceutical industry to understand aggregation pathways, and the risk factors that can induce aggregation, right from the start of the drug development process to align manufacturing processes with Quality by Design (QbD) principles. Given the link between protein aggregation and …
Archimedes - Particle Metrology System, Dynamic Light Scattering, Particle size, Resonant mass measurement, Sysmex FPIA-3000, Tech Talk, Zetasizer »
Recently this question was posed where a current dynamic light scattering (DLS) user inquired about detecting large but subvisible particles in a formulation:
“I have been looking at solutions from prefilled syringes which appear in flow microscopy to have significant levels of oligo-micron-sized silicone oil droplets. I don’t see any indication of these in my DLS measurements.”
With specifications for DLS systems like the Zetasizer from sub nanometer to several microns the initial concern is not unreasonable. However, on closer look the situation is not so apparent. The silicone oil droplets are …
Archimedes - Particle Metrology System, Dynamic Light Scattering, Molecular weight, Nanoparticle Tracking Analysis, Nanosight, Particle size, Resonant mass measurement, Tech Talk, Zeta potential, Zetasizer »
Having spent more than twenty years in the instrumentation arena and worked with a variety of different characterization techniques in lots of different application spaces, I have sometimes struggled to explain to my family what it is that I do. Particularly to my two young children who have never quite got the intricacies of nanomaterial characterization despite my attempts to stress the importance by referring to everyday products like computers and iPods that they take for granted.
So it was within this context that I found myself generating a presentation on the important and evolving sector of …
Archimedes - Particle Metrology System, Meet the Experts, Resonant mass measurement, Tech Talk »
There aren’t many things more pleasurable than a warm summer’s day in the Malvern Hills, but when you find out the mid-term meeting for your project is being held in Barcelona, it’s very difficult to be disappointed! Obviously Barcelona was an exciting prospect, but also being able to catch up with the UNION partners and see their progress in the last six months was something I was really looking forward to.
UNION is an EU funded project looking at developing nanoparticle assembly techniques and assembly monitoring technologies to prepare novel nanoclusters …
Archimedes - Particle Metrology System, Dynamic Light Scattering, Nanoparticle Tracking Analysis, Nanosight, Particle size, Resonant mass measurement, Tech Talk, Zetasizer »
PhD student Polly came into the lab on Monday morning to find that her purified protein had started to aggregate after a weekend in the fridge. The profile she got from her Dynamic Light Scattering (DLS) measurement clearly showed two peaks, with the second in the micron range. Her supervisor suggested she tried Nanoparticle Tracking Analysis (NTA) to get more information on the different sized aggregate species in her sample. This showed that there were aggregates of many different sizes present. The question was, what should she do next?
Thank you to everyone who joined us on 26th June, for the webinar “Protein Aggregation – Bridging the SubMicron Gap.” We held this webinar because we know that the formation of protein aggregates is a particular concern for parenteral administration biopharmaceuticals due to increased immunogenicity, and that as a consequence, companies increasingly need to monitor levels of subvisible particles present in therapeutic protein products initially and over the course of the shelf life. For subvisible particles between 0.1 – 10 µm this has, up until recently, been challenging. The webinar focused on the how the Archimedes instrument, which uses …Read the full story »
Archimedes - Particle Metrology System, Nanoparticle Tracking Analysis, Nanosight, Resonant mass measurement, Tech Talk »
Last week I attended the 4th Microbubble Symposium hosted by the Leeds Microbubble Consortium at University of Leeds in the UK. The meeting focused on the Fabrication, Characterization and Translational Applications of Microbubbles. I went along to hear about the latest developments in this fascinating area of research and to share my ideas on how our Nanosight & Archimedes instruments could help.
Much of the UK work on Microbubbles was shown to be coming from The University of Leeds where they are developing (amongst other things) “theranostic” Microbubbles that double as …
Archimedes - Particle Metrology System, Corporate, Dynamic Light Scattering, Malvern Events, Meet the Experts, Molecular weight, Resonant mass measurement, Size Exclusion Chromatography, Tech Talk, Viscotek, Zetasizer »
At Malvern, the launch of a new product is always an exciting time. With our stated goals of growing into the biopharmaceutical market to satisfy their development needs, the launch of a product to help us achieve this goal is particularly so.
That’s why we’re so excited about the upcoming launch of Archimedes. Archimedes is a novel system that can count particles in the size range of 50 nm to 5 µm using the technology of Resonant Mass Measurement (RMM). RMM delivers true size distributions based on the buoyant mass of …