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Size Exclusion Chromatography

Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

Archimedes, Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Image Analysis, Label-free analysis, MicroCal, Microcalorimetry, Morphologi G3-ID, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Rheology, Size Exclusion Chromatography, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Viscotek, Zetasizer »

[21 Sep 2017 | ]

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was a range of scientists and researchers from all over the world, with one common subject in mind: Protein Stability.

One of the highlights of this conference was the pre-conference workshop. This was a free workshop for all attendees of the conference, which was sponsored by Malvern Panalytical. The workshop included a range …

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Differential Scanning Calorimetry, Dynamic Light Scattering, Meet the Experts, MicroCal, Molecular size, Molecular weight, OMNISEC, Particle concentration, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Zetasizer »

[18 Jul 2017 | ]

Monoclonal Antibodies – or mAbs for short

Since many of these mAbs require refrigeration we had used dynamic light scattering (with a Zetasizer) to show the formation of aggregates after freeze-thaw cycles (for details see application note “Effect of storage conditions on Immunoglobulin“). These could be seen both in the increased polydispersity index (PDI) and the appearance of a large-size aggregate peak in the intensity size distribution as shown to the right. The area under the peak gives an indication of the presence of the large aggregates but is not suited for …

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Gel permeation chromatography, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk »

[23 Feb 2017 | ]

Recently, I’ve presented a new webinar focusing on the protein characterization using the OMNISEC, the highest quality Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system for the measurement of absolute molecular weight, molecular size, intrinsic viscosity, branching and other parameters. Thank you to all participants for your interest and attention during the webinar.
In this webinar, I highlighted the key features and benefits of the OMNISEC system. How to run samples and analyze data and how to access repeatability of multiple sample measurements.

As usual, after the live section of …

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Binding affinity and Kd, Differential Scanning Calorimetry, Dynamic Light Scattering, Français, Gel permeation chromatography, Image Analysis, Isothermal Titration Calorimetry, Laser Diffraction, Meet the Experts, Molecular size, Nanoparticle Tracking Analysis, Particle concentration, Particle size, Rheology, Rheometry, Size Exclusion Chromatography, Static Light Scattering, Zeta potential »

[21 Feb 2017 | ]

Afin de vous apporter un support concret pour utiliser votre système de façon optimale, finaliser vos bonnes pratiques de laboratoire et répondre à vos besoins spécifiques, Malvern organise des formations sur site ou en sessions dans nos 3 agences françaises (Orsay, Lyon et Toulouse).
Voici les dates de toutes les sessions programmées pour 2017 :
CARACTERISATION DES PARTICULES PAR DIFFRACTION LASER
Objectifs : Perfectionner les méthodes de caractérisation des particules en voie sèche et liquide, définir les bonnes pratiques de laboratoire et s’adapter aux besoins spécifiques de chaque installation. Comprendre le principe physique …

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Gel permeation chromatography, Malvern Panalytical Events, OMNISEC, Size Exclusion Chromatography »

[7 Feb 2017 | ]

Last year we asked you to tell us about your most interesting or challenging Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) sample and why it presented such a challenge. For instance, if you’ve made two polymers which should be different but you can’t tell apart; or have polymers that are so small you don’t have the sensitivity to measure accurately.
The top 3 submissions that presented the biggest challenge to our new OMNISEC advanced GPC/SEC system would be awarded with a full sample analysis along with a prize of …

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Dynamic Light Scattering, Gel permeation chromatography, Malvern Panalytical Events, Meet the Experts, Nanoparticle Tracking Analysis, Size Exclusion Chromatography, Zeta potential »

[26 Jan 2017 | ]

We will be attending Pittcon 2017 in Chicago, Illinois March 5th – 9th and once again we are pleased to feature a number of our recognized characterization experts who will be presenting a variety of short courses throughout the week.
Time is running out, so don’t miss out on the early bird deadline for discounted admission which ends on February 18th.
Below is a listing of our available short course offerings.
We look forward to seeing you in Chicago at one of our short courses and our Booth #2416.
Sunday, March 5, 2017 – 8:30 AM
Light Scattering …

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Gel permeation chromatography, Malvern Panalytical Events, Molecular size, Molecular weight, OMNISEC, Size Exclusion Chromatography, Viscotek »

[8 Dec 2016 | ]

Version Française
The French symposium of the French Group of Studies and Applications of Polymers (GFP) is the major French congress of polymers.
The 45th edition took place recently in Marseille under the bold architecture of the Villa Méditerranée, designed by Stefano Boeri, a committed actor of the Italian political and cultural scene.

During this conference, we presented the benefits of Viscotek SEC MALS 20.
The SEC-MALS 20 multi-angle light scattering detector  can be used on all GPC / SEC systems and especially Malvern’s latest OMNISEC range. The 20 signals of the SEC-MALS detectors connected …

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Français, Gel permeation chromatography, Malvern Panalytical Events, Molecular size, Molecular weight, OMNISEC, Size Exclusion Chromatography, Viscotek »

[8 Dec 2016 | ]

English version
Le colloque national du Groupe Français d’études et d’applications des Polymères (GFP) est le congrès français majeur des polymères.
La 45ème édition s’est déroulée récemment à Marseille sous l’architecture audacieuse de la Villa Méditerranée, conçue par Stefano Boeri, acteur engagé de la scène politique et culturelle italienne.

Durant ce congrès, nous avons présenté les avantages du Viscotek SEC MALS 20.
Le détecteur de diffusion de lumière multi-angle Viscotek SEC-MALS 20 peut être utilisé sur tous les systèmes de GPC/SEC et notamment sur le dernier OMNISEC de la gamme Malvern. Les signaux des 20 détecteurs du SEC-MALS …

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Gel permeation chromatography, Malvern Panalytical Events, Meet the Experts, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk, Viscotek »

[13 Sep 2016 | ]

Together with Sysmex, our distributor in Benelux, we are proud to be Gold Sponsors of the International Symposium on GPC/SEC and Related Techniques, taking place from 26-29th September in Amsterdam.
One of our technical experts, John Stenson, is looking forward to presenting some exciting new data in his talk “Combining multi-detector GPC measurements with ultra-high pressure GPC separations” at 3:30 pm on Wednesday 28th September, and says:
“This presentation will discuss the issues related to dispersion and how these can be minimized. We will then show the first high quality data from a combined multi-detector/ UPLC™ …

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Isothermal Titration Calorimetry, Meet the Experts, Nanoparticle Tracking Analysis, Rheometry, Size Exclusion Chromatography »

[5 Apr 2016 | ]

How time flies! Spring is here already! Just for you I have collated the latest list of our On Demand webinars. Just click and choose your favorite subject.
I also listed all our upcoming webinars for April. You can register for free and if you can’t attend, we’ll send you the recording few days after the live event so don’t hesitate, click now!
 
March On Demand webinars:

How Size Exclusion Chromatography can improve processes and quality in biopharmaceutical development
Applications of light scattering in pharmaceutical development
Interpretación de resultados de tamaño de partícula por la técnica de difracción …

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MicroCal, Size Exclusion Chromatography, Tech Talk »

[16 Feb 2016 | ]

Malvern is offering a two-day course designed to deliver comprehensive knowledge of Malvern’s Viscotek GPC/SEC technologies.
The course will be held in Malvern’s Houston, Texas facility from April 13-14, 2016 and will cover the theory behind the advanced detection options of Malvern’s GPC/SEC instruments, as well as a thorough presentation of the OmniSEC software (version 5.12 and lower). This ensures that users will leave the course with an understanding of all of the hardware and the software that their sample interacts with, and how to extract the most information from their …

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Gel permeation chromatography, Malvern Panalytical Events, Meet the Experts, OMNISEC, Size Exclusion Chromatography, Viscotek »

[1 Sep 2015 | ]

Malvern is offering a two-day course designed to deliver comprehensive knowledge of Malvern’s Viscotek GPC/SEC technologies. The course will be held in Malvern’s Houston, Texas facility from October 21-22, 2015 and will cover the theory behind the advanced detection options of Malvern’s GPC/SEC instruments, as well as a thorough presentation of the OmniSEC software. This ensures that users will leave the course with an understanding of all of the hardware and the software that their sample interacts with, and how to extract the most information from their sample.
Topics covered in …

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Molecular weight, Size Exclusion Chromatography, Static Light Scattering, Viscotek »

[6 Aug 2015 | ]

Looking over an old powerpoint file from Viscotek, an interesting slide on a unique application stood out. Investigating samples of the 20S Proteasome by light scattering in conjunction with refractive index RI was able to clearly determine whether a particular sample contained ‘filled or empty’ proteasomes.
In that case, both the empty and the loaded proteasome showed an RI peak at the same elution volume. Thus with conventional calibration, one would have had to conclude that these samples had the same molecular weight. However light scattering revealed that the filled proteasome …

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Gel permeation chromatography, Molecular size, Molecular structure, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk »

[14 Jul 2015 | ]

Multi-detector SEC – 10 reasons why protein scientists need one
In a previous post I listed the top ten reasons why a polymer characterization scientist might want to upgrade to multi-detector Gel Permeation Chromatography/Size Exclusion Chromatography, but there are similar points to be made as to why a protein characterization scientist might do the same.  We often focus on the benefits of our own systems but sometimes we may miss the benefits of the basic technique.
If you are a protein characterization scientist and you find yourself thinking, “My UV detector works …

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Gel permeation chromatography, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk »

[25 Mar 2015 | ]

A couple of weeks ago I had the opportunity to present a live webinar through Chemical & Engineering News about how our latest solution for Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC), OMNISEC, can help our customers with their synthetic polymer applications.
OMNISEC is a complete system for GPC/SEC measurements including a pump/degasser/autosampler module in OMNISEC RESOLVE and an advanced multi-detector module including RI, UV/Vis, Light scattering and IV detectors in OMNISEC REVEAL.
During the webinar we covered measurements from simple polymers like polystyrene to fascinating drug delivery polymers such as PLGA. At …

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Gel permeation chromatography, Molecular weight, Size Exclusion Chromatography, Static Light Scattering, Viscotek »

[10 Mar 2015 | ]

How to get the latest OmniSEC GPC software
One of the new developments at the Malvern exhibit at Pittcon is the OMNISEC system. If you are in New Orleans, come by our booth 3545 and take a look. We have already talked with quite a few existing users of our TDA and 270 systems. Did you know that the latest sofware version for our GPC / SEC systems is available for download from our website?
Where can I find and download the latest version of the OnniSEC software?
How to update your OmniSEC software:
Quick answer: OmniSEC software version 5.10
Did …

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Gel permeation chromatography, Malvern Panalytical Events, Molecular structure, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk »

[10 Mar 2015 | ]

I’m currently at Pittcon, the annual laboratory science conference and expo.  This year it’s in New Orleans, not too far from our factory in Houston.  Since New Orleans is known for its Cajun food and the Food Labs conference is going on at the same time, it made me think of the ways OMNISEC, our latest Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC) system can be used to support work in the food industry.
I’m sure you know that there are plenty of polysaccharides in processed foods but did you know how …

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Dynamic Light Scattering, Meet the Experts, Nanoparticle Tracking Analysis, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Size Exclusion Chromatography, Tech Talk »

[24 Feb 2015 | ]

I recently took part in a question and answer session with BioPharm International about techniques for measuring and characterizing protein aggregates. The full discussion can be viewed here, however I thought I would also use this blog post to summarize the main points.
Essentially, there is a pressing need within the biopharmaceutical industry to understand aggregation pathways, and the risk factors that can induce aggregation, right from the start of the drug development process to align manufacturing processes with Quality by Design (QbD) principles. Given the link between protein aggregation and …

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Gel permeation chromatography, Malvern Panalytical Events, Molecular structure, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk »

[19 Feb 2015 | ]

Hello everyone! This is my first blog so I think I should start by introducing myself.  My name is Amy Durrell and I am the Applications Scientist for the separation products here at Malvern Instruments.  My role is very varied as I am responsible for pre-sales sample analysis and I see all sorts of samples coming to the labs.
Recently I had the chance to be involved with the launch of our new complete multi-detection SEC system, OMNISEC.  This was my first product launch, so it is something I was very excited …

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Gel permeation chromatography, Molecular structure, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk »

[5 Feb 2015 | ]

GPC detector – OMNISEC – Your Questions Answered

Thanks so much to those of you who joined us for the launch of  OMNISEC, our new GPC/SEC system.  If you missed the event you can play it back by clicking here, to see how the new instrument can really enhance the characterization of polymers and proteins.  
Amy and I really enjoyed the launch and were thrilled by the interest it generated.  Many of you sent through questions during the event, and though I answered as many as possible inevitably there were too many to cover live.  As …

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