Ever heard of the terms “LALS”, “RALS” or MALS” and, if so, do you know what the difference between them is?
The answer is that these are all methods of measuring the molecular weight of proteins and polymers using light scattering. The technique involves detection of the intensity of the scattered light from a sample in conjunction with a concentration detector, where:
- LALS is Low Angle Light Scattering
- RALS is Right Angle Light Scattering
- MALS is Multiple Angle Light scattering
So, when is Low Angle Light Scattering (LALS) the right detection technique to use, and when do you need Multi Angle Light Scattering (MALS)?
Well, this is where a new white paper, published on the Malvern website that can help you. ‘Static Light Scattering Technologies For GPC/SEC Explained’ is a valuable guide for anyone assessing the value of a light scattering detector for gel permeation/size exclusion chromatography (GPC/SEC). This white paper explains the differences between all the light scattering techniques when used for GPC/SEC detection, including LALS, RALS and MALS, with a key focus on the practical uses of these different technologies and their respective advantages and disadvantages for different applications.