This is a screenshot of the OmniSEC profile of dextran. We offer a set of broad Dextran standards for light scattering and triple detection. The run conditions are detailed in the table below.

Run BufferPBS
Flow Rate [mL/min] 0.700
Column Set2 x A6000M
Column Temp (°C)35.0
Injection volume μL100.0
Concentration [mg/mL]2.97
Mw Weight Average [Da]73,123
Sample dn/dc [mL/g]0.137
Sample IV [dL/g]0.236
Chromatogram of Dextran: Refractive Index (RI), Viscosity, Right Angle Light Scattering (RALS) signals versus elution volume.

Chromatogram of Dextran: Refractive Index (RI), Viscosity, Right Angle Light Scattering (RALS) signals versus elution volume.

Dextran is a typical polysaccharide and chromatographs very well. This dextran has a broad polydispersity index PDI = Mw/Mn value of 1.38.  It is typically used as a system suitability sample for aqueous polymer applications, and often also a good choice for system calibration.

Previously

If you have any questions, please email me at ulf.nobbmann@malvern.com. Thanks!