On May 14, Malvern Panalytical is hosting a webinar “Isothermal Titration Calorimetry Enables Rapid Characterization of Enzyme Kinetics and Inhibition for the Human Soluble Epoxide Hydrolasepresented by Professor Maria R (Sasi) Conte and Dr. Giancarlo Abis, from Kings College, London UK.

Professor Conte’s team developed a fast, convenient and precise methodology using MicroCal PEAQ-ITC to elucidate the enzymatic properties of the soluble epoxide hydrolase (sEH) and characterize its inhibition. sEH catalyses epoxy lipid hydrolysis and is implicated in cardiovascular diseases, diabetes and pain.

Their results were recently published and reported by the King’s College London News Centre.

In this webinar, Professor Conte and Dr. Abis will describe the Isothermal Titration Calorimetry (ITC) method, present the data generated and discuss troubleshooting and optimization.

Register for the webinar now!

Enzyme kinetics and ITC

Every biochemical pathway depends on enzymes for catalysis, so the characterization of enzymes is key to biochemistry. Scientists study enzyme structure and how they work: mechanism of action, activity, specificity, inhibition and biological function.

Enzymes are also important in drug discovery and development since approximately half of the current drug targets are enzymes. Researchers are identifying and characterizing new enzymes for potential drug targets. Therapeutic enzymes are also biopharmaceutical drugs, used to replace enzymes that are missing or mutated due to genetic disorders.

Assays to study enzymatic activity and inhibition traditionally use labelled or tagged substrates, that have a change in the spectroscopic, fluorescent or chemical readout due to the enzymatic reaction. Frequently the enzyme of interest may not have a detectable readout, and the enzyme is coupled to other enzymatic reactions that have a readout.

Isothermal titration calorimetry (ITC) offers a direct method measuring the heat generated in an enzymatic reaction, a feature that is common to every catalyzed process. ITC has the potential to be applied to many biological systems that cannot be readily followed by other enzyme assay techniques. On July 7 and October 8, we will present other webinars focusing on the use of ITC to characterize enzymes, including data analysis. More information about these webinars will be released soon.

Further reading