Author Archives: Stoyan Milev

Stoyan Milev received his M.Sc. in Biochemostry in Sofia University (Sofia, Bulgaria); Ph.D. in Biochemisty from the University of Zurich (Zurich, Switzerland) and did post-doctoral training in the same institution. During his graduate studies he used MicroCal products extensively and became an expert in calorimetric data collection and analysis. Stoyan worked as a staff scientist for OLIS Inc. – a company manufacturing spectroscopic instruments based in Athens GA. In 2011 he joined MicroCal as Field Applications Specialist. Stoyan role is supporting the ITC and DSC application needs of customers on the Eastern part of North America.

Published 25 Aug 2020

DSC and protein stability: What does the enthalpy change mean?

DSC is the only analytical method that measures enthalpy change (∆H) of thermal transition directly. For protein, this is energy (heat) spent to unfold it.

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Published 17 Nov 2015

DSC and the meaning of Tm – DSC protein stability

We can extract multiple thermodynamic parameters from DSC data, such as ΔH, ΔHvH, ΔCp, and ΔG … but the most widely used parameter is Tm. Incidentally this is also the easiest and most accurate value to obtain – it is…READ MORE >

Published 23 Apr 2015

N-value ITC data

Don’t throw away your “bad” N-value ITC data The N-value commonly referred as the stoichiometry is often used as a qualifier for a “good” or “bad” titration. The reasoning goes like this – “if my N-value is 20% or more…READ MORE >

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