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Malvern Panalytical Events, Tech Talk, X-Ray Diffraction (XRD) »

[13 Nov 2018 | ]

Addressing concerns about data quality, space, throughput and user experience
More research institutions are moving towards a central laboratory model of shared analytical equipment. It encourages optimal usage of the technologies, building of expertise in techniques and ensuring the upkeep of better laboratory practices. X-ray diffraction (XRD) is a paramount technique for any material science research project. Therefore, the selection of a correct diffractometer model, suitable for the task, often comes to the top of the priority list, when designing a new laboratory.
As an Application Specialist at Malvern Panalytical, we often …

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Malvern Panalytical Events, Tech Talk »

[9 Nov 2018 | ]

About Sanjeeva Murthy and the New Jersey center for Biomaterials at Rutgers University
Sanjeeva Murthy is an Associate Research Professor at the New Jersey Center for Biomaterials (NJCBM), Rutgers University. He is a Materials Scientist with expertise in polymers, biomaterials and biological structures.  The NJCBM was founded in 1991 by Dr. Joachim Kohn with a mission to improve patient care and public health through the development and commercialization of future generations of biomaterials. Current activities in the laboratory include synthesis and characterization of new polymers for regenerative medicine, and fabricating them …

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Laser Diffraction, Mastersizer, Particle size, Tech Talk »

[1 Nov 2018 | ]

Are you pondering which laser diffraction particle size analyzer to use for your scientific research?
Many people consider the number of Google Scholar publication references as an indicator of acceptance of a particular scientific instrument’s technique, performance, and value. The higher the number of publications, the more credibility people tend to offer towards the choice of that instrument for their important research needs.
After doing a search on Google Scholar for references to the Mastersizer 3000 as well as the Mastersizer 2000, and then comparing versus several other models of laser diffraction …

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Differential Scanning Calorimetry, Dynamic Light Scattering, Electrophoretic light scattering, Empyrean, MicroCal, Nanosight, Particle concentration, Particle shape, Particle size, Tech Talk, X-Ray Diffraction (XRD), Zeta potential, Zetasizer »

[24 Oct 2018 | ]

Liposomes have been attractive delivery systems for decades due to their composition of natural biological lipids and structural resemblance to cell membranes suggesting metabolic compatibility, low toxicity, biocompatibility and lack of a strong immune response1.  They are comprised of spherical vesicles with an aqueous core enclosed by one or more phospholipid bilayers or lamellae and are frequently classified based on their size, polydispersity, and number of bilayers2. Control over these parameters has remained a challenge with most preparation methods and is further accentuated when moving from a laboratory to industrial …

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Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Electrophoretic light scattering, Elemental analysis, Elemental quantification, Empyrean, Epsilon, Image Analysis, Isothermal Titration Calorimetry, Laser Diffraction, Malvern Panalytical Events, Mastersizer, MicroCal, Microcalorimetry, Morphologi, Particle shape, Particle size, Tech Talk, X-Ray Diffraction (XRD), X-Ray Fluorescence (XRF), Zetasizer »

[19 Oct 2018 | ]

On October 2, 2018, we held a seminar entitled “Overcoming Characterization Challenges in Drug Formulation” in the Boston area.  It was a great opportunity to meet with many pharmaceutical scientists in the area and learn about their challenges and concerns.  The presentations addressed a number of topics of interest, from stability of formulations, trace phase and impurity detection, deformulation, root cause analysis, to optimizing small molecule candidate screening.  There were great questions from the audience, so we rounded up several questions and answers here to share.
How does the Mastersizer 3000 see …

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Binding affinity and Kd, Customer experience, Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Panalytical Events, MicroCal, Tech Talk »

[18 Oct 2018 | ]

The third European MicroCal meeting was held on 13th and 14th September in the beautiful venue of the Institute of Engineering & Technology (IET) at Savoy Place, on the banks of the River Thames in London. We welcomed delegates from across Europe to share and discuss their calorimetry expertise in an agenda packed with excellent scientific presentations.
Day 1
The meeting kicked off with a morning workshop which was repeated in the afternoon session. The workshop’s focus was firmly on data quality and the importance of establishing sample integrity prior to further characterization. This …

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Dynamic Light Scattering, Electrophoretic light scattering, Particle size, Tech Talk, Zeta potential, Zetasizer »

[15 Oct 2018 | ]

The new ZS Xplorer software is here!
Modern, flexible and different from the classic Zetasizer software, the novel ZS Xplorer interface was developed specifically for the latest generation of Zetasizer systems: the Zetasizer Pro and the Zetasizer Ultra. If you missed the launch, check out the exciting launch recording and Q&A session.
In order to help you easily adapt to the new interface, here are a few commonly encountered tips to help you become familiar with the entirely new software architecture. The main difference is that data are now saved in one …

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Dynamic Light Scattering, Electrophoretic light scattering, Malvern Panalytical Events, Meet the Experts, Molecular size, Molecular weight, Particle size, Protein Aggregation, Protein Mobility, Static Light Scattering, Tech Talk, Zeta potential, Zetasizer »

[12 Oct 2018 | ]

Join us for the next User group meeting!
User Group Meeting Announcement 19 Oct 2018 1pm-4pm EDT

We are pleased to announce the first virtual Zetasizer User Meeting ZUM-1! This initiative is an effort to foster the diffusion of knowledge and experience between our support group and those researchers that regularly evaluate light scattering data from the Zetasizer. The Zetasizer Users Community is invited to present and share data with specific questions. Get live feedback from our panel of experts.
The meeting will be live-streamed via Webex and is limited to 3.0 hours. …

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Kinexus, Rheology, Rheometry, Tech Talk »

[25 Sep 2018 | ]

So, which is it? I get asked this simple question more and more with the increased interest in Superpave. Superpave (SUperior PERforming Asphalt PAVEments) was developed by the Strategic Highway Research Program (SHRP) in the United States to help improve performance, efficiency, durability and safety of the highway system. As many more countries adopt Superpave or at least parts of it, there are many more individuals interested in understanding this new design system and all the new testing methods associated with it. Depending on what you search for, read in journals, …

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Binding affinity and Kd, Differential Scanning Calorimetry, MicroCal, Tech Talk »

[11 Sep 2018 | ]

This blog answers FAQs on the new Automated and Manual MicroCal PEAQ-DSC systems. For more information, check your user manual, or contact your regional Malvern Panalytical Support.
 
 
 
Q: How much sample do I need to fill the MicroCal PEAQ-DSC cell?
For the MicroCal Automated PEAQ-DSC, you need to have at least 325 μL of the sample or buffer in the appropriate well of the 96-well plate. This is the default setting for the experimental setup. 300 μL is removed by the filling syringe.
You can use a larger sample volume (up to 400 μL)  and …

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Borate fusion, Claisse, Tech Talk, X-Ray Fluorescence (XRF) »

[6 Sep 2018 | ]

Platinumware is used in sample preparation by fusion for XRF and ICP analysis. Crucibles (used for XRF and ICP analysis) and molds (only used for XRF) are made of 95% Pt and 5% Au.
Why use platinum and gold?
It has been proven that this alloy has a high melting point and can withstand extreme heat. Platinum has a great thermal conductivity and ensures very low contamination of the molten mixture during fusion. The addition of gold has a non-wetting effect. In short, the alloy of these two precious metals allows the …

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Crystal Structure Determination, Empyrean, Phase identification, Phase quantification, Tech Talk, X'Pert³, X-Ray Diffraction (XRD) »

[4 Sep 2018 | ]

Since I made my first steps in the powder diffraction community, I have been amazed by the many possibilities for collecting diffractograms. In this blog, I’ll describe how I learned about the advantages of transmission geometry over reflection geometry for specific samples.
In almost every powder diffractometer, the reflection geometry is being used: the detector is at the same side of the sample surface.
This geometry works very well for inorganic materials that can be prepared as flat samples with randomly oriented crystallites.
One of the disadvantages of reflection geometry is that it …

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Image Analysis, Morphologi, Tech Talk »

[28 Aug 2018 | ]

How the development of automated test scripts has increased test coverage and improved the overall quality of the software on the Morphologi 4 and 4-ID
My role as the Software Test Engineer of the Morphologi team is to ensure that changes made to the software meet the acceptance criteria and the high-quality standards of the overall software are still met. I find the work we do at Malvern Panalytical fascinating, however, testing software used for applications like Morphologi 4 and 4-ID has its challenges – the biggest of which is the time required to run …

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Gel permeation chromatography, Molecular structure, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk »

[22 Aug 2018 | ]

The most common detector in any GPC/SEC instrument is a refractive index (RI) detector.  There are multiple reasons for this, but the most important is because the difference in refractive index between that of a sample solution and that of the same solvent without a sample is directly proportional to the concentration of the sample.  This is why RI detectors are referred to as concentration detectors.  (It should be noted that UV-Vis detectors are also concentration detectors, but require the sample has a chromophore and absorbs light at a detectable …

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Differential Scanning Calorimetry, Label-free analysis, MicroCal, Tech Talk »

[14 Aug 2018 | ]

In a previous blog, I discussed best practices to achieve high-quality data with a MicroCal DSC. Here are more best practices for the MicroCal PEAQ-DSC system, as well as the MicroCal VP-Capillary DSC and VP-DSC systems. These best practices apply to the analysis of proteins in solution, and other biopolymers.
 
 
Sample requirements:

For high-quality Differential Scanning Calorimetry (DSC) data, the samples need to be in desired buffer (pH, salt, excipients, etc.), and the matched buffer is used in the DSC reference cell and for buffer-buffer scans.
Prepare samples in the desired buffer …

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Dynamic Light Scattering, Molecular size, Particle concentration, Particle size, Tech Talk, Zeta potential, Zetasizer »

[2 Aug 2018 | ]

 
Thanks to all who attended the launch webinar for Malvern Panalytical’s new Zetasizer systems, and special thanks to Mike Kaszuba for presenting a clear and interesting overview of the two instruments – we hope you all enjoyed it and found it interesting.

The launch of the Zetasizer Pro and Zetasizer Ultra received a great response and naturally, due to the innovations included in the new systems, lots of questions were sent to us both during and after the webinar. We’ve taken the opportunity to answer the most frequently-asked questions here, in …

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Borate fusion, LeDoser12, Tech Talk, X-Ray Fluorescence (XRF) »

[31 Jul 2018 | ]

The weighing step is of major importance in sample preparation by fusion since it has a direct impact on the analytical results.
Let’s see five best practices to ensure repeatability and productivity while weighing.
REPEATABILITY
Find the right weighing method
There are three weighing methods in sample preparation: sample-to-flux ratio, catch weight and manual weighing. The far best choice is sample-to-flux ratio since it ensures great repeatability and can be performed automatically using LeDoser-12 instrument. With this method, no software correction is required.
Avoid transferring the weighed content into a different container
You should always weigh …

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Gel permeation chromatography, Molecular weight, OMNISEC, Size Exclusion Chromatography, Tech Talk »

[12 Jul 2018 | ]

To many people, obtaining molecular weight information from GPC/SEC analysis involves a lengthy calibration process.  A series of standards of known molecular weight are analyzed and a calibration curve based on the standards’ retention volume is generated.  This can be a tedious procedure, and the result is only relative molecular weight data.
The inclusion of a light scattering detector in a GPC/SEC system eliminates the need to run a series of standards to generate a calibration curve, as the sample’s molecular weight is measured directly by the light scattering detector.  This …

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Dynamic Light Scattering, Tech Talk, Zeta potential, Zetasizer »

[10 Jul 2018 | ]

Dynamic light scattering (DLS) is widely used for ensemble characterization of hydrodynamic particle size, but I have a number of frustrations with the technique.
We measure our valuable samples in low concentrations and in very small volumes, so we use expensive, low volume, glass cells, which are optically superior to disposable plastic cuvettes, but we spend far too much time cleaning them, significantly reducing the productivity of our group.
Also, our samples range from monodisperse proteins to polymeric nanoparticles to liposomes, ranging from the nano-scale into the micro-scale and we need to …

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Dynamic Light Scattering, Particle size, Tech Talk, Zeta potential, Zetasizer »

[28 Jun 2018 | ]

What causes the most headaches for a particle characterization scientist?
There are many answers to this question, but one of the most obvious is dust or contaminants! As someone who regularly uses Dynamic Light Scattering (DLS) to measure particle size, the possibility of there being dust or contaminants in my sample is one of the things I always bear in mind.
The way I prepare my sample, the way I store my sample before measurement, remembering to put the measurement cap on the cuvette, but only after I’ve cleaned it using an …

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