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MicroCal

MicroCal microcalorimeters are powerful, analytical tools used to study biological systems. These include interactions between molecules as well as changes in conformation such as protein unfolding. The technique is very convenient requiring only minimal assay development and no labelling. The range includes both Differential Scanning and Isothermal Titration calorimetry.

Differential Scanning Calorimetry, Dynamic Light Scattering, Electrophoretic light scattering, Empyrean, MicroCal, Nanosight, Particle concentration, Particle shape, Particle size, Tech Talk, X-Ray Diffraction (XRD), Zeta potential, Zetasizer »

[24 Oct 2018 | ]

Liposomes have been attractive delivery systems for decades due to their composition of natural biological lipids and structural resemblance to cell membranes suggesting metabolic compatibility, low toxicity, biocompatibility and lack of a strong immune response1.  They are comprised of spherical vesicles with an aqueous core enclosed by one or more phospholipid bilayers or lamellae and are frequently classified based on their size, polydispersity, and number of bilayers2. Control over these parameters has remained a challenge with most preparation methods and is further accentuated when moving from a laboratory to industrial …

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Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Electrophoretic light scattering, Elemental analysis, Elemental quantification, Empyrean, Epsilon, Image Analysis, Isothermal Titration Calorimetry, Laser Diffraction, Malvern Panalytical Events, Mastersizer, MicroCal, Microcalorimetry, Morphologi, Particle shape, Particle size, Tech Talk, X-Ray Diffraction (XRD), X-Ray Fluorescence (XRF), Zetasizer »

[19 Oct 2018 | ]

On October 2, 2018, we held a seminar entitled “Overcoming Characterization Challenges in Drug Formulation” in the Boston area.  It was a great opportunity to meet with many pharmaceutical scientists in the area and learn about their challenges and concerns.  The presentations addressed a number of topics of interest, from stability of formulations, trace phase and impurity detection, deformulation, root cause analysis, to optimizing small molecule candidate screening.  There were great questions from the audience, so we rounded up several questions and answers here to share.
How does the Mastersizer 3000 see …

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Binding affinity and Kd, Customer experience, Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Panalytical Events, MicroCal, Tech Talk »

[18 Oct 2018 | ]

The third European MicroCal meeting was held on 13th and 14th September in the beautiful venue of the Institute of Engineering & Technology (IET) at Savoy Place, on the banks of the River Thames in London. We welcomed delegates from across Europe to share and discuss their calorimetry expertise in an agenda packed with excellent scientific presentations.
Day 1
The meeting kicked off with a morning workshop which was repeated in the afternoon session. The workshop’s focus was firmly on data quality and the importance of establishing sample integrity prior to further characterization. This …

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Binding affinity and Kd, Differential Scanning Calorimetry, MicroCal, Tech Talk »

[11 Sep 2018 | ]

This blog answers FAQs on the new Automated and Manual MicroCal PEAQ-DSC systems. For more information, check your user manual, or contact your regional Malvern Panalytical Support.
 
 
 
Q: How much sample do I need to fill the MicroCal PEAQ-DSC cell?
For the MicroCal Automated PEAQ-DSC, you need to have at least 325 μL of the sample or buffer in the appropriate well of the 96-well plate. This is the default setting for the experimental setup. 300 μL is removed by the filling syringe.
You can use a larger sample volume (up to 400 μL)  and …

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Differential Scanning Calorimetry, Label-free analysis, MicroCal, Tech Talk »

[14 Aug 2018 | ]

In a previous blog, I discussed best practices to achieve high-quality data with a MicroCal DSC. Here are more best practices for the MicroCal PEAQ-DSC system, as well as the MicroCal VP-Capillary DSC and VP-DSC systems. These best practices apply to the analysis of proteins in solution, and other biopolymers.
 
 
Sample requirements:

For high-quality Differential Scanning Calorimetry (DSC) data, the samples need to be in desired buffer (pH, salt, excipients, etc.), and the matched buffer is used in the DSC reference cell and for buffer-buffer scans.
Prepare samples in the desired buffer …

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Binding affinity and Kd, Customer experience, Isothermal Titration Calorimetry, MicroCal »

[5 Jul 2018 | ]

Isothermal Titration Calorimetry (ITC) is considered the ‘gold standard’ biophysical assay to determine the binding affinity/dissociation constant for any interaction involving proteins, nucleic acids, or lipids. ITC also determines the binding stoichiometry (related to the number of binding sites), and the binding thermodynamics.
MicroCal LLC, now part of Malvern Panalytical, launched the world’s first commercial ITC instrument, the OMEGA, in 1987 (there are several OMEGA ITCs still in use today!).  MicroCal then followed this success with the MCS ITC in 1992, the VP-ITC in 1998, and the iTC200 in 2007. In …

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Binding affinity and Kd, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Isothermal Titration Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry, Molecular size, OMNISEC, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Viscosizer TD, Viscotek, Zetasizer »

[13 Jun 2018 | ]

The time is fast arriving for the third in our highly successful series of European MicroCal meetings! This year the meeting is organized by Malvern Panalytical alongside a Scientific Committee of expert users from academia and industry, all with the support of the ARBRE-MOBIEU network. Our venue will be the prestigious Institute of Engineering and Technology (IET), within the historical Savoy Place, perched on the north bank of the River Thames, with spectacular views across London.
The event will focus on applications, best practice, advanced data analysis and all the latest developments in both isothermal …

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Aeris, Claisse, Epsilon, Kinexus, Malvern Panalytical Events, Mastersizer, MicroCal, Morphologi, Nanosight, Zetium »

[11 Apr 2018 | ]

This week I’m at Analytica in Munich surrounded by my colleagues from Malvern Panalytical. Even if it took me longer to reach the show because of the airline strikes, I’m happy to be talking to our customers and showing them our new products.
Our recently launched products the Morphologi 4 and the Epsilon 4 are taking pride of place at the front of the stand and there has been a lot of interest in them.
For the Morphologi 4 and 4-ID, I have enjoyed demonstrating it live to attendees at the exhibition. I have met current customers who use …

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Corporate, Differential Scanning Calorimetry, MicroCal »

[4 Apr 2018 | ]

The MicroCal PEAQ-DSC was one of 15 technologies awarded a 2017 Innovation Award presented by The Medicine Maker Magazine. This was the third year that The Medicine Maker magazine has awarded this honor reserved for new technologies that aid in pharmaceutical drug development and manufacturing. These technologies range from those that reduce production time to others that improve the safety of a drug in an effort to provide recognition to the vendors whose technologies allow for breakthroughs in the pharmaceutical industry.
PEAQ-DSC is praised alongside Adents’ Prodigi, a cloud-based serialization solution; …

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Differential Scanning Calorimetry, Meet the Experts, MicroCal »

[20 Mar 2018 | ]

Recently, Technology Network published an online article on Differential Scanning Calorimetry (DSC) This article discusses the basics of DSC and provides guidelines and technical tips for successful DSC experiments.
This article focuses on the new MicroCal PEAQ-DSC system from Malvern Panalytical.  This instrument provides high quality, reproducible DSC data.  The MicroCal PEAQ-DSC instrument is an useful addition to any lab that analyzes proteins, nucleic acids, lipids, and other biopolymers. DSC data are critical to the stability characterization of biotherapeutic drugs during development, to help choose the most stable drugs.
For more information on …

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Differential Scanning Calorimetry, Dynamic Light Scattering, MicroCal, Molecular size, Molecular structure, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[16 Nov 2017 | ]

Measuring aggregation temperature using the Zetasizer

In dynamic light scattering (DLS), the hydrodynamic size and size distribution of particles in solution can be obtained.  It may be of interest to examine this measurement as a function of time and temperature, or as a ‘trend’, as it is called in the Malvern Zetasizer software.  For biological protein samples, this temperature trend is particularly interesting: although at low temperatures a protein may be stable and show repeatable size (and scattering intensity) measurements, typically at some elevated temperature (Tagg), protein molecules will show a tendency …

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Binding affinity and Kd, Isothermal Titration Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[12 Oct 2017 | ]

Isothermal Titration Calorimetry
Molecular recognition is key in all biological processes and cellular responses. It is critical to characterize the structural and thermodynamic forces behind the recognition and specificity of macromolecular interactions in order to understand the molecular events in a cell. Several biophysical techniques (including fluorescence, circular dichroism, Fourier transform infrared spectroscopy, mass spectroscopy, surface plasmon resonance, and microscale thermophoresis) are used to characterize non-covalent molecular interactions.
Isothermal titration calorimetry (ITC) has become a popular and versatile bioanalytical method for the direct measurement of binding affinity between interacting molecules.  ITC instruments were …

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Archimedes, Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Image Analysis, Label-free analysis, MicroCal, Microcalorimetry, Morphologi, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Rheology, Size Exclusion Chromatography, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Viscotek, Zetasizer »

[21 Sep 2017 | ]

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was a range of scientists and researchers from all over the world, with one common subject in mind: Protein Stability.

One of the highlights of this conference was the pre-conference workshop. This was a free workshop for all attendees of the conference, which was sponsored by Malvern Panalytical. The workshop included a range …

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Binding affinity and Kd, Isothermal Titration Calorimetry, MicroCal, Tech Talk »

[12 Sep 2017 | ]

Binding Affinity
 
Understanding binding affinity is key to appreciation of the intermolecular interactions driving biological processes, structural biology, and structure-function relationships. It is also measured as part of the drug discovery process to help design drugs that bind their targets selectively and specifically.
We recently launched a new short video showcasing the MicroCal PEAQ-ITC and how it is an essential piece to any biological interaction analysis toolkit for measuring Binding affinity and stoichiometry.

The MicroCal PEAQ-ITC offers key benefits over other ITC systems including:
PEAQ-Expert: New easy-to-use software to guide your way includes built-in …

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Differential Scanning Calorimetry, Dynamic Light Scattering, Meet the Experts, MicroCal, Molecular size, Molecular weight, OMNISEC, Particle concentration, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Zetasizer »

[18 Jul 2017 | ]

Monoclonal Antibodies – or mAbs for short

Since many of these mAbs require refrigeration we had used dynamic light scattering (with a Zetasizer) to show the formation of aggregates after freeze-thaw cycles (for details see application note “Effect of storage conditions on Immunoglobulin“). These could be seen both in the increased polydispersity index (PDI) and the appearance of a large-size aggregate peak in the intensity size distribution as shown to the right. The area under the peak gives an indication of the presence of the large aggregates but is not suited for …

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Differential Scanning Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[4 Jul 2017 | ]

Protein Antigens and DSC

This blog highlights two recent articles that focus on the use of a MicroCal Differential Scanning Calorimetry (DSC) system.
Differential Scanning Calorimetry – A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
I.B. Duruwoju, K.M. Bhandal, J. Hu, B. Carpick, and M. Kirkitadze
Journal of Visualized Experiments (JoVE), Issue 121, e55262, doi:10.3791/55262 (2017)
The authors (from Sanofi Pasteur in Toronto, Canada), provide a detailed protocol to perform Differential Scanning Calorimetry experiments on an automated DSC, such as a MicroCal VP-Capillary DSC system from Malvern Instruments.  This piece is a JoVE …

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Archimedes, Customer experience, Differential Scanning Calorimetry, Gel permeation chromatography, Image Analysis, MicroCal, Morphologi, Nanoparticle Tracking Analysis, Nanosight, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Viscotek »

[1 Jun 2017 | ]

Our key driver at Malvern Instruments is to create technologies that make a difference or solve a problem, and working hand-in-hand with our customers enables us to make sure that’s exactly what’s happening, out in the field.  We place a high value on our collaborations and partnerships with customers from a wide range of industries and organizations across the globe and love to see how our systems are being used in real-world scenarios.  This gives us vital feedback on what we’re doing well, and also any tweaks we need to make in order to optimize …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, MicroCal, Microcalorimetry, Molecular weight, Tech Talk »

[23 May 2017 | ]

A professional and personal view
If your newsfeed from scientific and mainstream media sources is anything like mine, you have seen numerous articles and blogs about the ‘reproducibility crisis’ in the life sciences.
Well-established cornerstones of scientific inquiry and research are the publication of findings in peer-reviewed journals, along with the opportunity for others to reproduce and verify published data.  This advancement and consolidation of knowledge leads to further understanding on how biological systems work, and provides the basis for the discovery and development of new drugs which benefit all of us.
However, there …

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Archimedes, Chemical identification, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry, Molecular size, Molecular structure, Molecular weight, Morphologi, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Protein Mobility, Viscosizer TD, Zeta potential, Zetasizer »

[16 May 2017 | ]

With Pre-Conference Workshop on Biophysical Characterization and Particle Analysis
 

Date: July 17th – 20th.
We at Malvern know that protein stability is important in numerous disciplines, ranging from basic and medical biochemistry to pharmaceutical sciences. However, it is rare that researchers from all the relevant areas can join to discuss the critical issues in the field. The 2017 Colorado Protein Stability Conference provides a unique forum for this exchange of information. The topics to be covered include protein folding, dynamics and thermodynamic stability; amyloidosis and protein aggregation; and stability, formulation, analysis and processing …

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Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[11 May 2017 | ]

In September 2016 Malvern Instruments co-organized a European MicroCal meeting with Institut Pasteur and ARBRE European molecular biophysics network. The meeting was very successful and sparked animated discussions during scientific presentations, panel and poster sessions and interactive workshops.  A key focus was on best practice for experimental design and data analysis to ensure high-quality data and informed decisions that help drive scientific projects forward.
In two days, together with more than 120 participants from 19 countries, we covered a wide range of topics, from interaction and kinetic analysis with ITC to …

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