Home » Archive

Differential Scanning Calorimetry

Differential Scanning Calorimetry (DSC) is a technique used to characterize the stability of a protein or other biomolecule directly in its native form. It does this by measuring the heat change associated with the molecule’s thermal denaturation when heated at a constant rate.

Differential Scanning Calorimetry, Dynamic Light Scattering, Electrophoretic light scattering, Empyrean, MicroCal, Nanosight, Particle concentration, Particle shape, Particle size, Tech Talk, X-Ray Diffraction (XRD), Zeta potential, Zetasizer »

[24 Oct 2018 | ]

Liposomes have been attractive delivery systems for decades due to their composition of natural biological lipids and structural resemblance to cell membranes suggesting metabolic compatibility, low toxicity, biocompatibility and lack of a strong immune response1.  They are comprised of spherical vesicles with an aqueous core enclosed by one or more phospholipid bilayers or lamellae and are frequently classified based on their size, polydispersity, and number of bilayers2. Control over these parameters has remained a challenge with most preparation methods and is further accentuated when moving from a laboratory to industrial …

Read the full story »

Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Electrophoretic light scattering, Elemental analysis, Elemental quantification, Empyrean, Epsilon, Image Analysis, Isothermal Titration Calorimetry, Laser Diffraction, Malvern Panalytical Events, Mastersizer, MicroCal, Microcalorimetry, Morphologi, Particle shape, Particle size, Tech Talk, X-Ray Diffraction (XRD), X-Ray Fluorescence (XRF), Zetasizer »

[19 Oct 2018 | ]

On October 2, 2018, we held a seminar entitled “Overcoming Characterization Challenges in Drug Formulation” in the Boston area.  It was a great opportunity to meet with many pharmaceutical scientists in the area and learn about their challenges and concerns.  The presentations addressed a number of topics of interest, from stability of formulations, trace phase and impurity detection, deformulation, root cause analysis, to optimizing small molecule candidate screening.  There were great questions from the audience, so we rounded up several questions and answers here to share.
How does the Mastersizer 3000 see …

Read the full story »

Binding affinity and Kd, Customer experience, Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Malvern Panalytical Events, MicroCal, Tech Talk »

[18 Oct 2018 | ]

The third European MicroCal meeting was held on 13th and 14th September in the beautiful venue of the Institute of Engineering & Technology (IET) at Savoy Place, on the banks of the River Thames in London. We welcomed delegates from across Europe to share and discuss their calorimetry expertise in an agenda packed with excellent scientific presentations.
Day 1
The meeting kicked off with a morning workshop which was repeated in the afternoon session. The workshop’s focus was firmly on data quality and the importance of establishing sample integrity prior to further characterization. This …

Read the full story »

Binding affinity and Kd, Differential Scanning Calorimetry, MicroCal, Tech Talk »

[11 Sep 2018 | ]

This blog answers FAQs on the new Automated and Manual MicroCal PEAQ-DSC systems. For more information, check your user manual, or contact your regional Malvern Panalytical Support.
 
 
 
Q: How much sample do I need to fill the MicroCal PEAQ-DSC cell?
For the MicroCal Automated PEAQ-DSC, you need to have at least 325 μL of the sample or buffer in the appropriate well of the 96-well plate. This is the default setting for the experimental setup. 300 μL is removed by the filling syringe.
You can use a larger sample volume (up to 400 μL)  and …

Read the full story »

Differential Scanning Calorimetry, Label-free analysis, MicroCal, Tech Talk »

[14 Aug 2018 | ]

In a previous blog, I discussed best practices to achieve high-quality data with a MicroCal DSC. Here are more best practices for the MicroCal PEAQ-DSC system, as well as the MicroCal VP-Capillary DSC and VP-DSC systems. These best practices apply to the analysis of proteins in solution, and other biopolymers.
 
 
Sample requirements:

For high-quality Differential Scanning Calorimetry (DSC) data, the samples need to be in desired buffer (pH, salt, excipients, etc.), and the matched buffer is used in the DSC reference cell and for buffer-buffer scans.
Prepare samples in the desired buffer …

Read the full story »

Binding affinity and Kd, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Isothermal Titration Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry, Molecular size, OMNISEC, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Viscosizer TD, Viscotek, Zetasizer »

[13 Jun 2018 | ]

The time is fast arriving for the third in our highly successful series of European MicroCal meetings! This year the meeting is organized by Malvern Panalytical alongside a Scientific Committee of expert users from academia and industry, all with the support of the ARBRE-MOBIEU network. Our venue will be the prestigious Institute of Engineering and Technology (IET), within the historical Savoy Place, perched on the north bank of the River Thames, with spectacular views across London.
The event will focus on applications, best practice, advanced data analysis and all the latest developments in both isothermal …

Read the full story »

Corporate, Differential Scanning Calorimetry, MicroCal »

[4 Apr 2018 | ]

The MicroCal PEAQ-DSC was one of 15 technologies awarded a 2017 Innovation Award presented by The Medicine Maker Magazine. This was the third year that The Medicine Maker magazine has awarded this honor reserved for new technologies that aid in pharmaceutical drug development and manufacturing. These technologies range from those that reduce production time to others that improve the safety of a drug in an effort to provide recognition to the vendors whose technologies allow for breakthroughs in the pharmaceutical industry.
PEAQ-DSC is praised alongside Adents’ Prodigi, a cloud-based serialization solution; …

Read the full story »

Differential Scanning Calorimetry, Meet the Experts, MicroCal »

[20 Mar 2018 | ]

Recently, Technology Network published an online article on Differential Scanning Calorimetry (DSC) This article discusses the basics of DSC and provides guidelines and technical tips for successful DSC experiments.
This article focuses on the new MicroCal PEAQ-DSC system from Malvern Panalytical.  This instrument provides high quality, reproducible DSC data.  The MicroCal PEAQ-DSC instrument is an useful addition to any lab that analyzes proteins, nucleic acids, lipids, and other biopolymers. DSC data are critical to the stability characterization of biotherapeutic drugs during development, to help choose the most stable drugs.
For more information on …

Read the full story »

Differential Scanning Calorimetry, Dynamic Light Scattering, MicroCal, Molecular size, Molecular structure, Particle size, Protein Aggregation, Tech Talk, Zetasizer »

[16 Nov 2017 | ]

Measuring aggregation temperature using the Zetasizer

In dynamic light scattering (DLS), the hydrodynamic size and size distribution of particles in solution can be obtained.  It may be of interest to examine this measurement as a function of time and temperature, or as a ‘trend’, as it is called in the Malvern Zetasizer software.  For biological protein samples, this temperature trend is particularly interesting: although at low temperatures a protein may be stable and show repeatable size (and scattering intensity) measurements, typically at some elevated temperature (Tagg), protein molecules will show a tendency …

Read the full story »

Archimedes, Binding affinity and Kd, Chemical identification, Differential Scanning Calorimetry, Dynamic Light Scattering, Gel permeation chromatography, Image Analysis, Label-free analysis, MicroCal, Microcalorimetry, Morphologi, Nanoparticle Tracking Analysis, Nanosight, Particle concentration, Particle shape, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Rheology, Size Exclusion Chromatography, Taylor Dispersion Analysis, Tech Talk, Viscosizer TD, Viscotek, Zetasizer »

[21 Sep 2017 | ]

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was a range of scientists and researchers from all over the world, with one common subject in mind: Protein Stability.

One of the highlights of this conference was the pre-conference workshop. This was a free workshop for all attendees of the conference, which was sponsored by Malvern Panalytical. The workshop included a range …

Read the full story »

Differential Scanning Calorimetry, Dynamic Light Scattering, Meet the Experts, MicroCal, Molecular size, Molecular weight, OMNISEC, Particle concentration, Protein Aggregation, Size Exclusion Chromatography, Taylor Dispersion Analysis, Zetasizer »

[18 Jul 2017 | ]

Monoclonal Antibodies – or mAbs for short

Since many of these mAbs require refrigeration we had used dynamic light scattering (with a Zetasizer) to show the formation of aggregates after freeze-thaw cycles (for details see application note “Effect of storage conditions on Immunoglobulin“). These could be seen both in the increased polydispersity index (PDI) and the appearance of a large-size aggregate peak in the intensity size distribution as shown to the right. The area under the peak gives an indication of the presence of the large aggregates but is not suited for …

Read the full story »

Differential Scanning Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[4 Jul 2017 | ]

Protein Antigens and DSC

This blog highlights two recent articles that focus on the use of a MicroCal Differential Scanning Calorimetry (DSC) system.
Differential Scanning Calorimetry – A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
I.B. Duruwoju, K.M. Bhandal, J. Hu, B. Carpick, and M. Kirkitadze
Journal of Visualized Experiments (JoVE), Issue 121, e55262, doi:10.3791/55262 (2017)
The authors (from Sanofi Pasteur in Toronto, Canada), provide a detailed protocol to perform Differential Scanning Calorimetry experiments on an automated DSC, such as a MicroCal VP-Capillary DSC system from Malvern Instruments.  This piece is a JoVE …

Read the full story »

Archimedes, Customer experience, Differential Scanning Calorimetry, Gel permeation chromatography, Image Analysis, MicroCal, Morphologi, Nanoparticle Tracking Analysis, Nanosight, Particle size, Protein Aggregation, Raman spectroscopy, Resonant mass measurement, Viscotek »

[1 Jun 2017 | ]

Our key driver at Malvern Instruments is to create technologies that make a difference or solve a problem, and working hand-in-hand with our customers enables us to make sure that’s exactly what’s happening, out in the field.  We place a high value on our collaborations and partnerships with customers from a wide range of industries and organizations across the globe and love to see how our systems are being used in real-world scenarios.  This gives us vital feedback on what we’re doing well, and also any tweaks we need to make in order to optimize …

Read the full story »

Differential Scanning Calorimetry, Isothermal Titration Calorimetry, MicroCal, Microcalorimetry, Molecular weight, Tech Talk »

[23 May 2017 | ]

A professional and personal view
If your newsfeed from scientific and mainstream media sources is anything like mine, you have seen numerous articles and blogs about the ‘reproducibility crisis’ in the life sciences.
Well-established cornerstones of scientific inquiry and research are the publication of findings in peer-reviewed journals, along with the opportunity for others to reproduce and verify published data.  This advancement and consolidation of knowledge leads to further understanding on how biological systems work, and provides the basis for the discovery and development of new drugs which benefit all of us.
However, there …

Read the full story »

Differential Scanning Calorimetry, Isothermal Titration Calorimetry, Meet the Experts, MicroCal, Microcalorimetry »

[11 May 2017 | ]

In September 2016 Malvern Instruments co-organized a European MicroCal meeting with Institut Pasteur and ARBRE European molecular biophysics network. The meeting was very successful and sparked animated discussions during scientific presentations, panel and poster sessions and interactive workshops.  A key focus was on best practice for experimental design and data analysis to ensure high-quality data and informed decisions that help drive scientific projects forward.
In two days, together with more than 120 participants from 19 countries, we covered a wide range of topics, from interaction and kinetic analysis with ITC to …

Read the full story »

Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, MicroCal, Microcalorimetry »

[1 Mar 2017 | ]

The NEW MicroCal PEAQ-DSC – the gold standard for thermal stability determination
Differential scanning calorimetry (DSC) is an established biophysical tool for protein stability characterization during drug development. DSC data are used to advance the most stable and ‘developable’ proteins into the pipeline, and optimize the process and formulation conditions which will maintain protein stability during manufacturing and storage. DSC data are also important in higher order structure (HOS) protein characterization, lot-to-lot comparability, and characterization of biosimilar drugs compared to innovators.
Our previous technology, the MicroCal VP-Capillary DSC Automated system, is a …

Read the full story »

Binding affinity and Kd, Differential Scanning Calorimetry, Dynamic Light Scattering, Français, Gel permeation chromatography, Image Analysis, Isothermal Titration Calorimetry, Laser Diffraction, Meet the Experts, Molecular size, Nanoparticle Tracking Analysis, Particle concentration, Particle size, Rheology, Rheometry, Size Exclusion Chromatography, Static Light Scattering, Zeta potential »

[21 Feb 2017 | ]

Afin de vous apporter un support concret pour utiliser votre système de façon optimale, finaliser vos bonnes pratiques de laboratoire et répondre à vos besoins spécifiques, Malvern organise des formations sur site ou en sessions dans nos 3 agences françaises (Orsay, Lyon et Toulouse).
Voici les dates de toutes les sessions programmées pour 2017 :
CARACTERISATION DES PARTICULES PAR DIFFRACTION LASER
Objectifs : Perfectionner les méthodes de caractérisation des particules en voie sèche et liquide, définir les bonnes pratiques de laboratoire et s’adapter aux besoins spécifiques de chaque installation. Comprendre le principe physique …

Read the full story »

Differential Scanning Calorimetry, Malvern Panalytical Events, MicroCal, Tech Talk »

[14 Feb 2017 | ]

My introduction to Differential Scanning Calorimetry (DSC) was a postdoctoral fellow in the late 1980s.  I was producing mutant forms of the enzyme thymidylate synthase, and I needed to characterize the effects amino acid changes had on the overall stability of the protein.  Using a DSC instrument at our facility, I compared the thermal stability of the different mutants to wild-type protein by ranking the thermal transition temperature (TM). As I recall, I needed about 2 mg -4 mg protein per DSC experiment, which was a lot of protein at …

Read the full story »

Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry »

[2 Feb 2017 | ]

We are proud to announce Malvern Instruments’s Gold Sponsorship of Global Engage’s Biosimilars and Biologics Congress 2017.  This two-day event will be taking place on Monday 6th and Tuesday 7th March 2017, at the Leonardo Royal Hotel Berlin Alexanderplatz.
This meeting will be attended by industry, regulatory and academic experts from all areas of protein, antibody and biosimilar research and will comprise three topic streams: Innovations in Antibody-based Therapeutics; Expression, Purification and Characterization (with a particular focus on product stability and half-life); Biosimilars.
Natalia Markova, Principal Scientist, Malvern MicroCal, will be discussing …

Read the full story »

Differential Scanning Calorimetry, Label-free analysis, Malvern Panalytical Events, Meet the Experts, MicroCal, Microcalorimetry »

[31 Jan 2017 | ]

DSC for the regulated environment
 

 
Advancing biotherapeutic molecules along the drug development pipeline is a complex, time-consuming and expensive process. Good stability is key to a candidate’s progression, and must be assessed and reviewed throughout development – from candidate selection, through formulation development, all the way to batch-to-batch comparability or biosimilarity assessment….and beyond! Objective, reliable stability data is needed for confident decision-making about which drug products and processes to move through development into commercialization.
Differential Scanning Calorimetry (DSC) is a biophysical tool for protein stability characterization. The MicroCal VP-Capillary DSC system, launched in 2002, …

Read the full story »