Size Exclusion Chromatography (SEC)


Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

Published 25 Sep 2019

Rh or Rg: Which is best for my GPC/SEC samples?

The primary goal when employing gel permeation / size exclusion chromatography (GPC/SEC) is often to determine the molecular weight of a macromolecular sample.  Multi-detector systems, such as Malvern Panalytical’s OMNISEC and TDAmax systems, are able to offer a range of…READ MORE >

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Published 3 Sep 2019

Help! I need some…GPC/SEC expert advice

There are many things to consider when maintaining a GPC/SEC instrument: the mobile phase, the column set, the pump components, the post-column filter, and up to four different detectors…all in a single system! And that’s not even accounting for your…READ MORE >

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Published 13 Aug 2019

Can my GPC/SEC chromatogram be trusted?

You’ve done the hard parts to get your GPC/SEC instrument ready: prepared the mobile phase, stabilized the detector baselines, and prepared your sample.  After injection and completion of your sample sequence, you have acquired data.  Congrats!  Now, before you even…READ MORE >

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Published 20 Jun 2019

How does multi-detector GPC/SEC work?

Whether your samples are synthetic polymers, natural materials such as polysaccharides, or proteins, antibodies, or other biological samples, gel permeation / size exclusion chromatography (GPC/SEC) is the ideal technique for characterizing those and other types of macromolecules.  The information offered from…READ MORE >

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Published 30 May 2019

Using GPC/SEC for compositional analysis

Gel Permeation / Size Exclusion Chromatography is the ideal technique for determining the molecular weight of macromolecules.  In addition to molecular weight, other molecular parameters can be calculated by taking advantage of various detector combinations, such as intrinsic viscosity (IV),…READ MORE >

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