Size Exclusion Chromatography (SEC)


Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

Published 14 Jan 2020

Pittcon 2020 – Short courses on sizing and separations – in Chicago

  This year’s Pittsburgh Conference (or Pittcon for short) is taking place in Chicago, from March 1 – March 5, 2020. I noticed the earlier early bird time frame for Pittcon short courses. As in previous years, there is a…READ MORE >

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Published 25 Sep 2019

Rh or Rg: Which is best for my GPC/SEC samples?

The primary goal when employing gel permeation / size exclusion chromatography (GPC/SEC) is often to determine the molecular weight of a macromolecular sample.  Multi-detector systems, such as Malvern Panalytical’s OMNISEC and TDAmax systems, are able to offer a range of…READ MORE >

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Published 3 Sep 2019

Help! I need some…GPC/SEC expert advice

There are many things to consider when maintaining a GPC/SEC instrument: the mobile phase, the column set, the pump components, the post-column filter, and up to four different detectors…all in a single system! And that’s not even accounting for your…READ MORE >

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Published 13 Aug 2019

Can my GPC/SEC chromatogram be trusted?

You’ve done the hard parts to get your GPC/SEC instrument ready: prepared the mobile phase, stabilized the detector baselines, and prepared your sample.  After injection and completion of your sample sequence, you have acquired data.  Congrats!  Now, before you even…READ MORE >

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Published 20 Jun 2019

How does multi-detector GPC/SEC work?

Whether your samples are synthetic polymers, natural materials such as polysaccharides, or proteins, antibodies, or other biological samples, gel permeation / size exclusion chromatography (GPC/SEC) is the ideal technique for characterizing those and other types of macromolecules.  The information offered from…READ MORE >

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