Size Exclusion Chromatography (SEC)


Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

Published 22 Aug 2018

What is a dn/dc value and why is it important for GPC/SEC?

The most common detector in any GPC/SEC instrument is a refractive index (RI) detector.  There are multiple reasons for this, but the most important is because the difference in refractive index between that of a sample solution and that of…READ MORE >

Published 24 Jul 2018

Why does my viscometer signal have a negative peak?

If you’ve ever analyzed GPC/SEC data collected on your OMNISEC or TDA 305 instrument that included a viscometer detector, you might have observed and wondered about a negative peak that elutes in the viscometer signal long after the sample peak. …READ MORE >

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Published 12 Jul 2018

Why do I need a standard if I have a GPC/SEC system with a light scattering detector?

To many people, obtaining molecular weight information from GPC/SEC analysis involves a lengthy calibration process.  A series of standards of known molecular weight are analyzed and a calibration curve based on the standards’ retention volume is generated.  This can be…READ MORE >

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Published 13 Jun 2018

3rd European MicroCal meeting – Join us in London this September for 2 days of analytical bioscience!

The time is fast arriving for the third in our highly successful series of European MicroCal meetings! This year the meeting is organized by Malvern Panalytical alongside a Scientific Committee of expert users from academia and industry, all with the support of…READ MORE >

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Published 5 Dec 2017

WINN – Innovation for pharmaceutical products

It was my great pleasure to present as part of the Worcestershire Innovation Network at their WINN Wednesday event on the 22nd of November, showcasing the best in innovation across Worcestershire. This included a local company which makes artificial kneecaps and hips, an…READ MORE >

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