Size Exclusion Chromatography (SEC)


Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.

Published 5 Dec 2017

WINN – Innovation for pharmaceutical products

It was my great pleasure to present as part of the Worcestershire Innovation Network at their WINN Wednesday event on the 22nd of November, showcasing the best in innovation across Worcestershire. This included a local company which makes artificial kneecaps and hips, an…READ MORE >

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Published 23 Oct 2017

Polydispersity – what does it mean for DLS and chromatography?

The term polydispersity (or more recently dispersity without the poly, as per IUPAC recommendation) is used to describe the degree of “non-uniformity” of a distribution.  In the field of molecular/nanoparticular characterization, there are in principle two different definitions of polydispersity,…READ MORE >

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Published 21 Sep 2017

“Two hundred scientists walked into a bar…”

I know this sounds like the beginning of a bad joke, but in this case, it was actually the beginning of the 2017 Colorado Protein Stability Conference, held in July at Beaver Run Resort in Breckenridge, Colorado. In attendance was…READ MORE >

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Published 18 Jul 2017

Impact of Freeze -Thaw on monoclonal Antibody solutions

Monoclonal Antibodies – or mAbs for short Since many of these mAbs require refrigeration we had used dynamic light scattering (with a Zetasizer) to show the formation of aggregates after freeze-thaw cycles (for details see application note “Effect of storage conditions…READ MORE >

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Published 23 Feb 2017

OMNISEC for Protein Characterization – Demo at your desk Q&A

Recently, I’ve presented a new webinar focusing on the protein characterization using the OMNISEC, the highest quality Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system for the measurement of absolute molecular weight, molecular size, intrinsic viscosity, branching and…READ MORE >

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